6I07

Crystal structure of EpCAM in complex with scFv

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Single chain Fv	polymer	262	27857.9	2		Homo sapiens (Human)
2	C, D (C, D)	Epithelial cell adhesion molecule	polymer	253	28622.4	2	UniProt (P16422) Pfam (PF18635) Pfam (PF00086) Pfam (PF21283)	Homo sapiens (Human)	Ep-CAM,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein,EGP,Epithelial glycoprotein 314,hEGP314,KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1
3	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F, G, H, I (A, B, C, D)	water	water		18.0	117	Chemie (HOH)

Sequence modifications

C, D: 25 - 265 (UniProt: P16422)

PDB	External Database	Details
Gln 74	Asn 74	engineered mutation
Gln 111	Asn 111	engineered mutation
Gln 198	Asn 198	engineered mutation
Gly 266	-	expression tag
Gly 267	-	expression tag
Gly 268	-	expression tag
Gly 269	-	expression tag
Ser 270	-	expression tag
His 271	-	expression tag
His 272	-	expression tag
His 273	-	expression tag
His 274	-	expression tag
His 275	-	expression tag
His 276	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	112960.5
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	113052.6

*Water molecules are not included.