6HWH

Structure of a functional obligate respiratory supercomplex from Mycobacterium smegmatis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ubiquinol-cytochrome c reductase iron-sulfur subunit	polymer	408	44869.4	2	UniProt (A0R051) Pfam (PF19297) Pfam (PF00355) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
2	G, C	Co-purified unknown transmembrane helices built as polyALA	polymer	74	6315.8	2		Mycobacterium smegmatis MC2 155
3	H, D	Co-purified unknown transmembrane helices built as polyALA	polymer	65	5549.8	2		Mycobacterium smegmatis MC2 155
4	I, E	Co-purified unknown peptide built as polyALA	polymer	20	1720.1	2		Mycobacterium smegmatis MC2 155
5	J, F	Co-purified unknown peptide built as polyALA	polymer	35	2996.7	2		Mycobacterium smegmatis MC2 155
6	M, K, i, j	Cytochrome bc1 complex cytochrome c subunit	polymer	268	27874.5	4	UniProt (A0R050) Pfam (PF00034) Pfam (PF13442) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
7	P, L	Cytochrome c oxidase subunit 2	polymer	341	38077.5	2	UniProt (A0R057) Pfam (PF00116) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
8	R, N	MSMEG_4693	polymer	79	8365.5	2	UniProt (A0R1B6) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
9	T, O	Uncharacterized protein MSMEG_4692/MSMEI_4575	polymer	157	15911.0	2	UniProt (A0R1B5) Pfam (PF17174) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
10	V, Q	Cytochrome c oxidase subunit 1	polymer	583	64841.6	2	UniProt (A0R0M4) Pfam (PF00115) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
11	X, S	Cytochrome c oxidase polypeptide 4	polymer	139	15177.4	2	UniProt (A0R056) Pfam (PF12270) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	Cytochrome aa3 subunit 4,Cytochrome c oxidase polypeptide IV
12	Z, W	Cytochrome c oxidase subunit 3	polymer	203	22196.9	2	UniProt (A0R049) Pfam (PF00510) In PDB	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
13	b, Y	Ubiquinol-cytochrome C reductase QcrB	polymer	554	61271.9	2	UniProt (I7FGS8) Pfam (PF13631) UniProt (by SIFTS) (A0R052) In PDB	Mycobacterium smegmatis MC2 155
14	A, B	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
15	Y, M, V, b, K...	CARDIOLIPIN	non-polymer		1464.0	8	Chemie (CDL)
16	Y, M, b	MENAQUINONE-9	non-polymer		785.2	4	Chemie (MQ9)
17	P, V, L, Q	COPPER (II) ION	non-polymer		63.5	6	Chemie (CU)
18	V, Q	HEME-AS	non-polymer		921.0	4	Chemie (HAS)
19	i, j, V	HEME C	non-polymer		618.5	4	Chemie (HEC)
20	Y, b	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	4	Chemie (HEM)

Sequence modifications

b, Y: 1 - 546 (UniProt: I7FGS8)

PDB	External Database	Details
Asp 547	-	expression tag
Tyr 548	-	expression tag
Lys 549	-	expression tag
Asp 550	-	expression tag
Asp 551	-	expression tag
Asp 552	-	expression tag
Asp 553	-	expression tag
Lys 554	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	686085.5
Non-Polymers*	Number of molecules	32
	Total formula weight	24210.0
All*	Total formula weight	710295.5

*Water molecules are not included.