6HTR

Yeast 20S proteasome with human beta2c (S171G) in complex with 13

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, O	PROTEASOME SUBUNIT ALPHA TYPE-2	polymer	250	27191.8	2	UniProt (P23639) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B, P	Proteasome subunit alpha type-3	polymer	258	28748.2	2	UniProt (P23638) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit Y13,Multicatalytic endopeptidase complex subunit Y13,Proteasome component Y13,Proteinase YSCE subunit 13
3	C, Q	PROTEASOME SUBUNIT ALPHA TYPE-4	polymer	254	28478.1	2	UniProt (P40303) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
4	D, R	PROTEASOME SUBUNIT ALPHA TYPE-5	polymer	260	28649.1	2	UniProt (P32379) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
5	E, S	PROTEASOME SUBUNIT ALPHA TYPE-6	polymer	234	25634.0	2	UniProt (P40302) Pfam (PF00227) Pfam (PF10584) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
6	F, T	Probable proteasome subunit alpha type-7	polymer	288	31575.1	2	UniProt (P21242) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit C1,Multicatalytic endopeptidase complex subunit C1,Proteasome component C1,Proteinase YSCE subunit 1
7	G, U	PROTEASOME SUBUNIT ALPHA TYPE-1	polymer	252	28033.8	2	UniProt (P21243) Pfam (PF10584) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
8	H, V	Proteasome subunit beta type-7	polymer	234	25292.0	2	UniProt (Q99436) Pfam (PF00227) Pfam (PF12465) In PDB	Homo sapiens (Human)	Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z
9	I, W	Proteasome subunit beta type-3	polymer	205	22627.8	2	UniProt (P25451) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PUP3,Multicatalytic endopeptidase complex subunit PUP3,Proteasome component PUP3
10	J, X	PROTEASOME SUBUNIT BETA TYPE-4	polymer	198	22545.7	2	UniProt (P22141) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
11	K, Y	Proteasome subunit beta type-5	polymer	212	23325.2	2	UniProt (P30656) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE2,Multicatalytic endopeptidase complex subunit PRE2,Proteasome component PRE2,Proteinase YSCE subunit PRE2
12	L, Z	Proteasome subunit beta type-6	polymer	222	24883.9	2	UniProt (P23724) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Multicatalytic endopeptidase complex subunit C5,Proteasome component C5
13	M, a	Proteasome subunit beta type-7	polymer	246	27200.9	2	UniProt (P30657) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Macropain subunit PRE4,Multicatalytic endopeptidase complex subunit PRE4,Proteasome component PRE4,Proteinase YSCE subunit PRE4
14	N, b	PROTEASOME SUBUNIT BETA TYPE-1	polymer	196	21517.2	2	UniProt (P38624) Pfam (PF00227) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
15	G, H, I, K, N...	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
16	H, K, V, Y	(2~{S})-~{N}-[(2~{S})-1-[[(2~{S})-1-[4-(aminomethyl)phenyl]-4-methylsulfonyl-butan-2-yl]amino]-3-oxidanyl-1-oxidanylidene-propan-2-yl]-2-[[(2~{S})-2-azido-3-phenyl-propanoyl]amino]-4-methyl-pentanamide	non-polymer		629.8	4	Chemie (GQT)
17		water	water		18.0	697	Chemie (HOH)

Sequence modifications

H, V: 1 - 234 (UniProt: Q99436)

PDB	External Database	Details
Gly 171	Ser 214	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	731405.8
Non-Polymers*	Number of molecules	12
	Total formula weight	2713.5
All*	Total formula weight	734119.3

*Water molecules are not included.