6HMS

Cryo-EM map of DNA polymerase D from Pyrococcus abyssi in complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase II small subunit	polymer	400	45078.0	1	UniProt (Q9V2F3) Pfam (PF00149) In PDB	Pyrococcus abyssi	Pol II,Exodeoxyribonuclease small subunit
2	B	DNA polymerase II large subunit,DNA polymerase II large subunit	polymer	1270	144419.0	1	UniProt (Q9V2F4) Pfam (PF03833) In PDB	Pyrococcus abyssi	Pol II,Exodeoxyribonuclease large subunit,Pol II,Exodeoxyribonuclease large subunit
3	P	DNA (5'-D(*GP*AP*GP*AP*CP*GP*GP*GP*CP*CP*GP*CP*GP*TP*C)-3')	polymer	15	4636.0	1		Pyrococcus abyssi
4	T	DNA (5'-D(P*TP*GP*AP*CP*GP*CP*GP*GP*CP*CP*CP*GP*TP*CP*TP*C)-3')	polymer	16	4851.1	1		Pyrococcus abyssi
5	A	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
6	A, B	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

A: 225 - 619 (UniProt: Q9V2F3)

PDB	External Database	Details
Tyr 197	-	expression tag
Glu 198	-	expression tag
Val 199	-	expression tag
Lys 200	-	expression tag
Phe 201	-	expression tag
Ala 451	His 451	conflict

B: 1 - 955 (UniProt: Q9V2F4)
B: 956 - 1270 (UniProt: Q9V2F4)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	198984.1
Non-Polymers*	Number of molecules	5
	Total formula weight	317.5
All*	Total formula weight	199301.6

*Water molecules are not included.