6HAA
Structure of a covalent complex of endo-Xyloglucanase from Cellvibrio japonicus after reacting with XXXG(2F)-beta-DNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cellulase, putative, cel5D | polymer | 396 | 44338.1 | 2 | UniProt (B3PD52) Pfam (PF00150) | Cellvibrio japonicus (Pseudomonas fluorescens subsp. cellulosa) | |
| 2 | C (C) | beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-glucopyranose | branched | 932.8 | 1 | In PDB GlyTouCan (G07540IS) | |||
| 3 | D (D) | alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-[alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-2-deoxy-2-fluoro-alpha-D-glucopyranose | branched | 1064.9 | 1 | In PDB GlyTouCan (G06479TB) | |||
| 4 | E, F, K (A, B) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 5 | G, L (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 6 | H, I (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 2 | Chemie (PGE) | |||
| 7 | J, M (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 8 | N (B) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 9 | O, P (A, B) | water | water | 18.0 | 357 | Chemie (HOH) |
Sequence modifications
A, B: 96 - 468 (UniProt: B3PD52)
| PDB | External Database | Details |
|---|---|---|
| Met 73 | - | initiating methionine |
| Gly 74 | - | expression tag |
| Ser 75 | - | expression tag |
| Ser 76 | - | expression tag |
| His 77 | - | expression tag |
| His 78 | - | expression tag |
| His 79 | - | expression tag |
| His 80 | - | expression tag |
| His 81 | - | expression tag |
| His 82 | - | expression tag |
| Ser 83 | - | expression tag |
| Ser 84 | - | expression tag |
| Gly 85 | - | expression tag |
| Leu 86 | - | expression tag |
| Val 87 | - | expression tag |
| Pro 88 | - | expression tag |
| Arg 89 | - | expression tag |
| Gly 90 | - | expression tag |
| Ser 91 | - | expression tag |
| His 92 | - | expression tag |
| Met 93 | - | expression tag |
| Ala 94 | - | expression tag |
| Ser 95 | - | expression tag |
| Ala 255 | Glu 255 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 88676.3 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1997.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1397.5 | |
| All* | Total formula weight | 92071.5 |
