6H6X
Structure of an evolved dimeric form of the UbiD-class enzyme HmfF from Pelotomaculum thermopropionicum in complex with prFMN
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | 3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases | polymer | 448 | 48881.4 | 2 | UniProt (A5D4Z9) Pfam (PF20695) Pfam (PF01977) Pfam (PF20696) | Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI) | |
| 2 | C, G (B, A) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 3 | D, H (B, A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 4 | E, I (B, A) | 1-deoxy-5-O-phosphono-1-(3,3,4,5-tetramethyl-9,11-dioxo-2,3,8,9,10,11-hexahydro-7H-quinolino[1,8-fg]pteridin-12-ium-7-y l)-D-ribitol | non-polymer | 525.5 | 2 | Chemie (4LU) | |||
| 5 | F, J (B, A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | K, L (B, A) | water | water | 18.0 | 183 | Chemie (HOH) |
Sequence modifications
B, A: 1 - 448 (UniProt: A5D4Z9)
| PDB | External Database | Details |
|---|---|---|
| Asn 315 | Ala 315 | engineered mutation |
| Arg 348 | Asn 348 | engineered mutation |
| Asp 351 | Phe 351 | engineered mutation |
| Asn 355 | Thr 355 | engineered mutation |
| Pro 388 | Ala 388 | engineered mutation |
| Thr 393 | Phe 393 | engineered mutation |
| Phe 395 | Val 395 | engineered mutation |
| Arg 399 | Met 399 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 97762.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1319.2 | |
| All* | Total formula weight | 99081.9 |
