6H6X

Structure of an evolved dimeric form of the UbiD-class enzyme HmfF from Pelotomaculum thermopropionicum in complex with prFMN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	3-polyprenyl-4-hydroxybenzoate decarboxylase and related decarboxylases	polymer	448	48881.4	2	UniProt (A5D4Z9) Pfam (PF20695) Pfam (PF01977) Pfam (PF20696) In PDB	Pelotomaculum thermopropionicum (strain DSM 13744 / JCM 10971 / SI)
2	B, A	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
3	B, A	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	B, A	1-deoxy-5-O-phosphono-1-(3,3,4,5-tetramethyl-9,11-dioxo-2,3,8,9,10,11-hexahydro-7H-quinolino[1,8-fg]pteridin-12-ium-7-y l)-D-ribitol	non-polymer		525.5	2	Chemie (4LU)
5	B, A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6		water	water		18.0	183	Chemie (HOH)

Sequence modifications

B, A: 1 - 448 (UniProt: A5D4Z9)

PDB	External Database	Details
Asn 315	Ala 315	engineered mutation
Arg 348	Asn 348	engineered mutation
Asp 351	Phe 351	engineered mutation
Asn 355	Thr 355	engineered mutation
Pro 388	Ala 388	engineered mutation
Thr 393	Phe 393	engineered mutation
Phe 395	Val 395	engineered mutation
Arg 399	Met 399	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97762.8
Non-Polymers*	Number of molecules	8
	Total formula weight	1319.2
All*	Total formula weight	99081.9

*Water molecules are not included.