6GXN
Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 23S ribosomal RNA | polymer | 2903 | 941321.3 | 1 | GenBank (1036415628) | Escherichia coli | |
| 2 | B (B) | 5S ribosomal RNA | polymer | 120 | 38813.1 | 1 | GenBank (1373146531) | Escherichia coli | |
| 3 | C (C) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 4 | D (D) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 5 | E (E) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli | Large ribosomal subunit protein uL4 |
| 6 | F (F) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli | Large ribosomal subunit protein uL5 |
| 7 | G (G) | 50S ribosomal protein L6 | polymer | 176 | 18801.6 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli | Large ribosomal subunit protein uL6 |
| 8 | H (H) | 50S ribosomal protein L9 | polymer | 149 | 15789.0 | 1 | UniProt (P0A7R1) Pfam (PF01281) Pfam (PF03948) | Escherichia coli | Large ribosomal subunit protein bL9 |
| 9 | I (I) | 50S ribosomal protein L11 | polymer | 141 | 14763.2 | 1 | UniProt (P0A7J7) Pfam (PF03946) Pfam (PF00298) | Escherichia coli | Large ribosomal subunit protein uL11 |
| 10 | J (J) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli | Large ribosomal subunit protein uL13 |
| 11 | K (K) | 50S ribosomal protein L14 | polymer | 122 | 13451.9 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli | Large ribosomal subunit protein uL14 |
| 12 | L (L) | 50S ribosomal protein L15 | polymer | 143 | 14877.3 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli | Large ribosomal subunit protein uL15 |
| 13 | M (M) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 14 | N (N) | 50S ribosomal protein L17 | polymer | 120 | 13721.9 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli | Large ribosomal subunit protein bL17 |
| 15 | O (O) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli | Large ribosomal subunit protein uL18 |
| 16 | P (P) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli | Large ribosomal subunit protein bL19 |
| 17 | Q (Q) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli | Large ribosomal subunit protein bL20 |
| 18 | R (R) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli | Large ribosomal subunit protein bL21 |
| 19 | S (S) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli | Large ribosomal subunit protein uL22 |
| 20 | T (T) | 50S ribosomal protein L23 | polymer | 93 | 10546.5 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli | Large ribosomal subunit protein uL23 |
| 21 | U (U) | 50S ribosomal protein L24 | polymer | 102 | 11078.9 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli | Large ribosomal subunit protein uL24 |
| 22 | V (V) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli | Large ribosomal subunit protein bL25 |
| 23 | W (W) | 50S ribosomal protein L27 | polymer | 75 | 8174.4 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli | Large ribosomal subunit protein bL27 |
| 24 | X (X) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli | Large ribosomal subunit protein bL28 |
| 25 | Y (Y) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli | Large ribosomal subunit protein uL29 |
| 26 | Z (Z) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli | Large ribosomal subunit protein uL30 |
| 27 | AA (0) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli | Large ribosomal subunit protein bL32 |
| 28 | BA (1) | 50S ribosomal protein L33 | polymer | 50 | 5814.8 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli | Large ribosomal subunit protein bL33 |
| 29 | CA (2) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli | Large ribosomal subunit protein bL34 |
| 30 | DA (3) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 31 | EA (4) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 32 | FA (5) | 50S ribosomal protein L10 | polymer | 131 | 14112.3 | 1 | UniProt (P0A7J3) Pfam (PF00466) | Escherichia coli | 50S ribosomal protein L8,Large ribosomal subunit protein uL10 |
| 33 | GA (7) | mRNA | polymer | 7 | 2229.4 | 1 | Escherichia coli | ||
| 34 | HA (a) | 16S ribosomal RNA | polymer | 1539 | 498725.4 | 1 | GenBank (1338015392) | Escherichia coli | |
| 35 | IA (b) | 30S ribosomal protein S2 | polymer | 218 | 24253.9 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli | Small ribosomal subunit protein uS2 |
| 36 | JA (c) | 30S ribosomal protein S3 | polymer | 206 | 23078.8 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli | Small ribosomal subunit protein uS3 |
| 37 | KA (d) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli | Small ribosomal subunit protein uS4 |
| 38 | LA (e) | 30S ribosomal protein S5 | polymer | 157 | 16532.1 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli | Small ribosomal subunit protein uS5 |
| 39 | MA (f) | 30S ribosomal protein S6 | polymer | 100 | 11669.4 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli | Small ribosomal subunit protein bS6 |
| 40 | NA (g) | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli | Small ribosomal subunit protein uS7 |
| 41 | OA (h) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli | Small ribosomal subunit protein uS8 |
| 42 | PA (i) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli | Small ribosomal subunit protein uS9 |
| 43 | QA (j) | 30S ribosomal protein S10 | polymer | 98 | 11197.0 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli | Small ribosomal subunit protein uS10 |
| 44 | RA (k) | 30S ribosomal protein S11 | polymer | 116 | 12388.1 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli | Small ribosomal subunit protein uS11 |
| 45 | SA (l) | 30S ribosomal protein S12 | polymer | 123 | 13637.0 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli | Small ribosomal subunit protein uS12 |
| 46 | TA (m) | 30S ribosomal protein S13 | polymer | 114 | 12625.8 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli | Small ribosomal subunit protein uS13 |
| 47 | UA (n) | 30S ribosomal protein S14 | polymer | 101 | 11546.4 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli | Small ribosomal subunit protein uS14 |
| 48 | VA (o) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli | Small ribosomal subunit protein uS15 |
| 49 | WA (p) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli | Small ribosomal subunit protein bS16 |
| 50 | XA (q) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli | Small ribosomal subunit protein uS17 |
| 51 | YA (r) | 30S ribosomal protein S18 | polymer | 65 | 7606.8 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli | Small ribosomal subunit protein bS18 |
| 52 | ZA (s) | 30S ribosomal protein S19 | polymer | 79 | 9057.6 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli | Small ribosomal subunit protein uS19 |
| 53 | AB (t) | 30S ribosomal protein S20 | polymer | 85 | 9506.2 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli | Small ribosomal subunit protein bS20 |
| 54 | BB (u) | 30S ribosomal protein S21 | polymer | 65 | 7763.1 | 1 | UniProt (P68679) Pfam (PF01165) | Escherichia coli | Small ribosomal subunit protein bS21 |
| 55 | CB (v) | Peptide chain release factor RF1 | polymer | 350 | 39488.1 | 1 | UniProt (P0A7I0) Pfam (PF03462) Pfam (PF00472) | Escherichia coli | RF-1 |
| 56 | DB (w) | Peptide chain release factor RF3 | polymer | 529 | 59651.9 | 1 | UniProt (P0A7I4) Pfam (PF00009) Pfam (PF03144) Pfam (PF16658) | Escherichia coli | RF-3 |
| 57 | EB (x) | fMet-tRNA | polymer | 77 | 24802.8 | 1 | GenBank (1387059712) | Escherichia coli | |
| 58 | FB (z) | Apidaecin | polymer | 14 | 1699.1 | 1 | UniProt (Q8WSY8) Pfam (PF00807) | Apis mellifera | |
| 59 | GB (w) | PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER | non-polymer | 521.2 | 1 | Chemie (GCP) |
Sequence modifications
A: 1 - 2903 (GenBank: 1036415628)
B: 1 - 119 (GenBank: 1373146531)
n: 1 - 101 (UniProt: P0AG59)
v: 2 - 351 (UniProt: P0A7I0)
z: 5 - 18 (UniProt: Q8WSY8)
| PDB | External Database | Details |
|---|---|---|
| C 747 | U 1155325 | conflict |
| G 1847 | A 1156425 | conflict |
| PDB | External Database | Details |
|---|---|---|
| A 120 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 35 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Cys 167 | Ser 167 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Arg 10 | Gln 23 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 58 |
| Total formula weight | 2261206.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 521.2 | |
| All* | Total formula weight | 2261727.5 |
