6GUT

CRYSTAL STRUCTURE OF NON-TYPEABLE HAEMOPHILUS INFLUENZAE PROTEIN E AND PILA EXPRESSED AS A SINGLE-CHAIN CHIMERIC PROTEIN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	23S rRNA pseudouridine synthase D,PilA	polymer	262	28753.2	2	UniProt (A4MX90) UniProt (Q5D8E3) Pfam (PF16747) In PDB	Haemophilus influenzae
2	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
3	A, B	ACETATE ION	non-polymer		59.0	3	Chemie (ACT)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5		water	water		18.0	628	Chemie (HOH)

Sequence modifications

A, B: 1 - 142 (UniProt: A4MX90)

PDB	External Database	Details
Glu 135	Lys 153	conflict
Gly 143	-	linker
Gly 144	-	linker

A, B: 145 - 254 (UniProt: Q5D8E3)

PDB	External Database	Details
Gly 255	-	expression tag
Gly 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	57506.3
Non-Polymers*	Number of molecules	9
	Total formula weight	749.5
All*	Total formula weight	58255.9

*Water molecules are not included.