6GUM

Structure of the A.thaliana E1 UFD domain in complex with E2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SUMO-conjugating enzyme SCE1	polymer	180	20180.9	1	UniProt (Q42551) Pfam (PF00179)	Arabidopsis thaliana (thale cress)	Protein EMBRYO DEFECTIVE 1637,Protein hus5 homolog,SUMO-conjugating enzyme 1,AtSCE1
2	B (B)	SAE2	polymer	209	23341.0	1	UniProt (A0A178VMI9) Pfam (PF14732) UniProt (by SIFTS) (Q9SJT1)	Arabidopsis thaliana (thale cress)
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E (A, B)	water	water		18.0	64	Chemie (HOH)

Sequence modifications

A: 1 - 160 (UniProt: Q42551)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: 437 - 625 (UniProt: A0A178VMI9)

PDB	External Database	Details
Met 417	-	initiating methionine
Gly 418	-	expression tag
Ser 419	-	expression tag
Ser 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
Ser 427	-	expression tag
Ser 428	-	expression tag
Gly 429	-	expression tag
Leu 430	-	expression tag
Val 431	-	expression tag
Pro 432	-	expression tag
Arg 433	-	expression tag
Gly 434	-	expression tag
Ser 435	-	expression tag
His 436	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43521.9
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	43614.0

*Water molecules are not included.