6GPK

Crystal structure of human GDP-D-mannose 4,6-dehydratase (E157Q) in complex with GDP-Man

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	GDP-mannose 4,6 dehydratase	polymer	373	42527.1	4	UniProt (O60547) Pfam (PF16363)	Homo sapiens (Human)	GDP-D-mannose dehydratase,GMD
2	CA, DA, E, EA, F... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	28	Chemie (EDO)
3	AA, IA, NA, R (B, C, D, A)	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		743.4	4	Chemie (NAP)
4	BA, JA, OA, S (B, C, D, A)	GUANOSINE-5'-DIPHOSPHATE-ALPHA-D-MANNOSE	non-polymer		605.3	4	Chemie (GDD)
5	HA (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	PA, QA, RA, SA (A, B, C, D)	water	water		18.0	858	Chemie (HOH)

Sequence modifications

A, B, C, D: 23 - 372 (UniProt: O60547)

PDB	External Database	Details
Met 0	-	initiating methionine
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
Ser 7	-	expression tag
Ser 8	-	expression tag
Gly 9	-	expression tag
Val 10	-	expression tag
Asp 11	-	expression tag
Leu 12	-	expression tag
Gly 13	-	expression tag
Thr 14	-	expression tag
Glu 15	-	expression tag
Asn 16	-	expression tag
Leu 17	-	expression tag
Tyr 18	-	expression tag
Phe 19	-	expression tag
Gln 20	-	expression tag
Ser 21	-	expression tag
Met 22	-	expression tag
Gln 157	Glu 157	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	170108.6
Non-Polymers*	Number of molecules	37
	Total formula weight	7225.0
All*	Total formula weight	177333.5

*Water molecules are not included.