6GOS

E. coli Microcin synthetase McbBCD complex with pro-MccB17 bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Bacteriocin microcin B17	polymer	77	6853.4	1	UniProt (P05834) In PDB	Escherichia coli str. K-12 substr. MG1655	MccB17
2	1, 2	Microcin B17-processing protein McbB	polymer	295	34032.4	2	UniProt (P23184) In PDB	Escherichia coli str. K-12 substr. MG1655
3	C	Microcin B17-processing protein McbC	polymer	272	30789.1	1	UniProt (P23185) Pfam (PF00881) In PDB	Escherichia coli str. K-12 substr. MG1655
4	D	Microcin B17-processing protein McbD	polymer	396	44964.0	1	UniProt (P23186) Pfam (PF02624) In PDB	Escherichia coli str. K-12 substr. MG1655
5	1, 2	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6	1	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
7	1, D	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
8	2, D	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
9	C	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	1	Chemie (FMN)
10	D	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
11		water	water		18.0	385	Chemie (HOH)

Sequence modifications

A: 1 - 60 (UniProt: P05834)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Otz 39	Gly 39	modified residue
Otz 39	Ser 40	modified residue
Otz 39	Cys 41	modified residue
F75 45	Gly 47	modified residue
F75 45	Cys 48	modified residue
Toz 47	Gly 50	modified residue
Toz 47	Cys 51	modified residue
Toz 47	Ser 52	modified residue
Toz 49	Gly 54	modified residue
Toz 49	Cys 55	modified residue
Toz 49	Ser 56	modified residue
F6n 54	Gly 61	modified residue
F6n 54	Ser 62	modified residue
F6n 56	Gly 64	modified residue
F6n 56	Ser 65	modified residue

D: 1 - 396 (UniProt: P23186)

PDB	External Database	Details
Arg 171	Thr 171	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	150671.2
Non-Polymers*	Number of molecules	13
	Total formula weight	1218.6
All*	Total formula weight	151889.8

*Water molecules are not included.