6GMH

Structure of activated transcription complex Pol II-DSIF-PAF-SPT6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RPB1	polymer	1970	217610.0	1	Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) UniProt (by SIFTS) (I3LJR4) In PDB	Sus scrofa (Pig)
2	B	DNA-directed RNA polymerase subunit beta	polymer	1174	134041.4	1	UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB	Sus scrofa (Pig)
3	C	RNA polymerase II subunit C	polymer	275	31439.1	1	UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) In PDB	Sus scrofa (Pig)
4	D	RNA polymerase II subunit D	polymer	142	16331.3	1	UniProt (A0A287ADR4) Pfam (PF03874) In PDB	Sus scrofa (Pig)
5	E	RNA polymerase II subunit E	polymer	210	24644.3	1	UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) In PDB	Sus scrofa (Pig)
6	F	RNA polymerase II subunit F	polymer	127	14477.0	1	UniProt (F1SKN8) Pfam (PF01192) UniProt (by SIFTS) (A0A4X1VEK9) In PDB	Sus scrofa (Pig)
7	G	RNA polymerase II subunit G	polymer	172	19314.3	1	UniProt (I3LJZ9) In PDB	Sus scrofa (Pig)
8	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	150	17162.3	1	UniProt (I3LCB2) Pfam (PF03870) In PDB	Sus scrofa (Pig)
9	I	DNA-directed RNA polymerase II subunit RPB9	polymer	125	14541.2	1	UniProt (P60899) Pfam (PF02150) Pfam (PF01096) In PDB	Sus scrofa (Pig)	RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5
10	J	RPB10	polymer	67	7655.1	1		Sus scrofa (Pig)
11	K	RPB11	polymer	117	13310.3	1	UniProt (F1RKE4) Pfam (PF13656) In PDB	Sus scrofa (Pig)
12	L	RPB12	polymer	58	7018.2	1	UniProt (I3LN51) Pfam (PF03604) UniProt (by SIFTS) (A0A4X1TRS6) In PDB	Sus scrofa (Pig)
13	M	Transcription elongation factor SPT6,Transcription elongation factor SPT6,Transcription elongation factor SPT6	polymer	1726	198992.4	1	UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF22706) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633) In PDB	Homo sapiens (Human)	hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein,hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein,hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein
14	N	Non-template DNA	polymer	48	14932.5	1		synthetic construct
15	P	RNA	polymer	46	14843.9	1		synthetic construct
16	Q	CTR9,RNA polymerase-associated protein CTR9 homolog,RNA polymerase-associated protein CTR9 homolog	polymer	1178	125713.3	1	UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181) In PDB	Homo sapiens	SH2 domain-binding protein 1,SH2 domain-binding protein 1
17	T	Template DNA	polymer	48	14672.3	1		synthetic construct
18	U	LEO1,LEO1,RNA polymerase-associated protein LEO1	polymer	776	84875.8	1	UniProt (Q8WVC0) Pfam (PF04004) In PDB	Homo sapiens	Replicative senescence down-regulated leo1-like protein
19	V	PAF1,RNA polymerase II-associated factor 1 homolog,RNA polymerase II-associated factor 1 homolog	polymer	613	67031.3	1	UniProt (Q8N7H5) Pfam (PF03985) In PDB	Homo sapiens	hPAF1,Pancreatic differentiation protein 2,hPAF1,Pancreatic differentiation protein 2
20	W	WD repeat-containing protein 61	polymer	305	33617.5	1	UniProt (Q9GZS3) Pfam (PF00400) In PDB	Homo sapiens (Human)	Meiotic recombination REC14 protein homolog,SKI8 homolog,Ski8
21	X	CDC73	polymer	16	1379.7	1		Homo sapiens
22	Y	Transcription elongation factor SPT4	polymer	121	13508.5	1	UniProt (P63272) Pfam (PF06093) In PDB	Homo sapiens (Human)	hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit
23	Z	Transcription elongation factor SPT5	polymer	1087	121145.5	1	UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) In PDB	Homo sapiens (Human)	hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein
24	B, C, I, J, L...	ZINC ION	non-polymer		65.4	9	Chemie (ZN)
25	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

M: 1 - 1326 (UniProt: Q7KZ85)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

M: 1327 - 1334 (PDB: 6GMH)
M: 1338 - 1726 (UniProt: Q7KZ85)
Q: 1 - 300 (PDB: 6GMH)
Q: 301 - 1173 (UniProt: Q6PD62)

PDB	External Database	Details
Glu 1174	-	expression tag
Asn 1175	-	expression tag
Leu 1176	-	expression tag
Tyr 1177	-	expression tag
Gln 1178	-	expression tag

U: 371 - 529 (PDB: 6GMH)
U: 530 - 1195 (UniProt: Q8WVC0)
V: 198 - 338 (PDB: 6GMH)
V: 339 - 869 (UniProt: Q8N7H5)
Y: 1 - 117 (UniProt: P63272)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	1208257.1
Non-Polymers*	Number of molecules	10
	Total formula weight	613.0
All*	Total formula weight	1208870.1

*Water molecules are not included.