6GKH

CryoEM structure of the MDA5-dsRNA filament in complex with ADP-AlF4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Interferon-induced helicase C domain-containing protein 1	polymer	1007	114214.5	1	UniProt (Q8R5F7) Pfam (PF16739) Pfam (PF00619) Pfam (PF04851) Pfam (PF18119) Pfam (PF00271) Pfam (PF11648)	Mus musculus (Mouse)	Helicase with 2 CARD domains,Helicard,Interferon induced with helicase C domain protein 1,Melanoma differentiation-associated protein 5,MDA-5,RIG-I-like receptor 2,RLR-2
2	B (X)	RNA (5'-R(P*GP*UP*CP*AP*AP*GP*CP*CP*GP*AP*GP*GP*AP*GP*A)-3')	polymer	15	4894.0	1		Pseudomonas phage phi6
3	C (Y)	RNA (5'-R(P*UP*CP*UP*CP*CP*UP*CP*GP*GP*CP*UP*UP*GP*AP*C)-3')	polymer	15	4681.8	1		Pseudomonas phage phi6
4	D (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5	E (A)	TETRAFLUOROALUMINATE ION	non-polymer		103.0	1	Chemie (ALF)
6	F (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	G (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 1025 (UniProt: Q8R5F7)

PDB	External Database	Details
-	Asp 646	deletion
-	Lys 647	deletion
-	Ser 648	deletion
-	Asp 649	deletion
-	Asp 650	deletion
-	Glu 651	deletion
-	Ala 652	deletion
-	Ser 653	deletion
-	Ser 654	deletion
-	Cys 655	deletion
-	Asn 656	deletion
-	Asp 657	deletion
-	Gln 658	deletion
-	Leu 659	deletion
-	Lys 660	deletion
-	Gly 661	deletion
-	Asp 662	deletion
-	Val 663	deletion

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	123790.3
Non-Polymers*	Number of molecules	4
	Total formula weight	619.9
All*	Total formula weight	124410.2

*Water molecules are not included.