6GI3

Structure of Lytic Transglycosylase MltE mutant S73A from E.coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Endo-type membrane-bound lytic murein transglycosylase A	polymer	194	21398.3	1	UniProt (P0C960) Pfam (PF01464)	Escherichia coli	Peptidoglycan lytic endotransglycosylase
2	B (B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS)
3	C (B)	water	water		18.0	287	Chemie (HOH)

Sequence modifications

B: 19 - 203 (UniProt: P0C960)

PDB	External Database	Details
Met 18	-	initiating methionine
Ala 73	Ser 73	engineered mutation
Leu 204	-	expression tag
Glu 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	21398.3
Non-Polymers*	Number of molecules	1
	Total formula weight	122.1
All*	Total formula weight	21520.5

*Water molecules are not included.