6GHY

Structure of Lytic Transglycosylase MltE inactive mutant E64Q from E.coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	Endo-type membrane-bound lytic murein transglycosylase A	polymer	194	21413.3	2	UniProt (P0C960) Pfam (PF01464) In PDB	Escherichia coli (strain K12)	Peptidoglycan lytic endotransglycosylase
2	B, A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	3	Chemie (PEG)
3		water	water		18.0	295	Chemie (HOH)

Sequence modifications

B, A: 19 - 203 (UniProt: P0C960)

PDB	External Database	Details
Met 18	-	initiating methionine
Gln 64	Glu 64	engineered mutation
Leu 204	-	expression tag
Glu 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42826.7
Non-Polymers*	Number of molecules	3
	Total formula weight	318.4
All*	Total formula weight	43145.0

*Water molecules are not included.