6GF8

Molecular basis of egg coat filament cross-linking: structure of the glycosylated ZP1 ZP-N1 domain homodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Zona pellucida sperm-binding protein 1,Zona pellucida sperm-binding protein 1	polymer	136	15485.5	2	UniProt (A0A140JXP0) Pfam (PF22821)	Gallus gallus (Chicken)
2	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
3	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	E, F, G, H (A, B)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	4	Chemie (MPD)

Sequence modifications

A, B: 24 - 139 (UniProt: A0A140JXP0)

PDB	External Database	Details
Asp 19	-	expression tag
Ala 20	-	expression tag
Ala 21	-	expression tag
Gln 22	-	expression tag
Pro 23	-	expression tag
Gln 121	Asn 121	engineered mutation

A, B: 146 - 154 (UniProt: A0A140JXP0)

PDB	External Database	Details
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag
His 143	-	expression tag
His 144	-	expression tag
His 145	-	expression tag
Ser 154	Gly 149	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30971.0
Branched	Number of molecules	2
	Total formula weight	994.9
Non-Polymers*	Number of molecules	4
	Total formula weight	472.7
All*	Total formula weight	32438.6

*Water molecules are not included.