6GEZ
THE STRUCTURE OF TWITCH-2B N532F
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Green fluorescent protein,Optimized Ratiometric Calcium Sensor,Green fluorescent protein,Green fluorescent protein | polymer | 556 | 62483.0 | 2 | UniProt (P42212) UniProt (W5IDB2) Pfam (PF01353) Pfam (PF13499) | Aequorea victoria (Jellyfish) | |
| 2 | C, D, I, J (A, B) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 3 | E, F, G, H, K (A, B) | FORMIC ACID | non-polymer | 46.0 | 5 | Chemie (FMT) | |||
| 4 | L, M (A, B) | water | water | 18.0 | 78 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 228 (UniProt: P42212)
A, B: 236 - 304 (UniProt: W5IDB2)
A, B: 312 - 376 (UniProt: P42212)
A, B: 383 - 556 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Val 2 | - | insertion |
| Leu 65 | Phe 64 | engineered mutation |
| Crf 66 | Ser 65 | chromophore |
| Crf 66 | Tyr 66 | chromophore |
| Crf 66 | Gly 67 | chromophore |
| Ala 73 | Ser 72 | engineered mutation |
| Ala 146 | Tyr 145 | engineered mutation |
| Ile 147 | Asn 146 | engineered mutation |
| His 148 | Ser 147 | engineered mutation |
| Gly 149 | His 148 | engineered mutation |
| Thr 154 | Met 153 | engineered mutation |
| Ala 164 | Val 163 | engineered mutation |
| Gly 167 | Lys 166 | engineered mutation |
| Leu 168 | Ile 167 | engineered mutation |
| Asn 169 | Arg 168 | engineered mutation |
| Cys 170 | His 169 | engineered mutation |
| Lys 207 | Ala 206 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Phe 249 | Lys 14 | conflict |
| Val 300 | Met 65 | conflict |
| Pro 305 | - | linker |
| Ile 306 | - | linker |
| Tyr 307 | - | linker |
| Pro 308 | - | linker |
| Glu 309 | - | linker |
| Leu 310 | - | linker |
| Met 311 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Arg 229 | - | linker |
| Met 230 | - | linker |
| Gln 231 | - | linker |
| Val 232 | - | linker |
| Ala 233 | - | linker |
| Asp 234 | - | linker |
| Ala 235 | - | linker |
| Gly 313 | Ser 175 | engineered mutation |
| Tyr 341 | Thr 203 | engineered mutation |
| Lys 344 | Ala 206 | engineered mutation |
| Leu 369 | His 231 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 377 | - | linker |
| Gly 378 | - | linker |
| Thr 379 | - | linker |
| Gly 380 | - | linker |
| Gly 381 | - | linker |
| Ser 382 | - | linker |
| Val 384 | - | insertion |
| Arg 413 | Ser 30 | engineered mutation |
| Asn 422 | Tyr 39 | engineered mutation |
| Leu 429 | Phe 46 | engineered mutation |
| Leu 447 | Phe 64 | engineered mutation |
| Cr2 448 | Ser 65 | chromophore |
| Cr2 448 | Tyr 66 | chromophore |
| Cr2 448 | Gly 67 | chromophore |
| Leu 451 | Val 68 | engineered mutation |
| Met 452 | Gln 69 | engineered mutation |
| Ala 455 | Ser 72 | engineered mutation |
| Phe 532 | Asn 149 | engineered mutation |
| Thr 536 | Met 153 | engineered mutation |
| Ala 546 | Val 163 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 124965.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 390.4 | |
| All* | Total formula weight | 125356.4 |
