6GAM
Structure of E14Q variant of E. coli hydrogenase-2 (as-isolated enzyme)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | S, T | Hydrogenase-2 small chain | polymer | 301 | 32744.9 | 2 | UniProt (P69741) Pfam (PF01058) Pfam (PF14720) In PDB | Escherichia coli (strain K12) | HYD2,Membrane-bound hydrogenase 2 small subunit,NiFe hydrogenase |
2 | L, M | Hydrogenase-2 large chain | polymer | 567 | 62556.9 | 2 | UniProt (P0ACE0) Pfam (PF00374) In PDB | Escherichia coli (strain K12) | HYD2,Membrane-bound hydrogenase 2 large subunit,NiFe hydrogenase |
3 | S, T | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
4 | S, T | FE3-S4 CLUSTER | non-polymer | 295.8 | 2 | Chemie (F3S) | |||
5 | L, M | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 2 | Chemie (FCO) | |||
6 | L, M | NICKEL (II) ION | non-polymer | 58.7 | 2 | Chemie (NI) | |||
7 | L, M | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
8 | water | water | 18.0 | 1068 | Chemie (HOH) |
Sequence modifications
S, T: 1 - 293 (UniProt: P69741)
L, M: 1 - 567 (UniProt: P0ACE0)
PDB | External Database | Details |
---|---|---|
Arg 294 | - | expression tag |
Ser 295 | - | expression tag |
His 296 | - | expression tag |
His 297 | - | expression tag |
His 298 | - | expression tag |
His 299 | - | expression tag |
His 300 | - | expression tag |
His 301 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gln 14 | Glu 14 | variant |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 190603.6 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 2435.9 | |
All* | Total formula weight | 193039.5 |