6G94
Structure of E. coli hydrogenase-1 C19G variant in complex with cytochrome b
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (S, T, Q, R) | Hydrogenase-1 small chain | polymer | 335 | 36768.6 | 4 | UniProt (P69739) Pfam (PF01058) Pfam (PF14720) | Escherichia coli K-12 | HYD1,Membrane-bound hydrogenase 1 small subunit,NiFe hydrogenase |
| 2 | B, D, F, H (L, M, J, K) | Hydrogenase-1 large chain | polymer | 582 | 64751.4 | 4 | UniProt (P0ACD8) Pfam (PF00374) | Escherichia coli (strain K12) | HYD1,Membrane-bound hydrogenase 1 large subunit,NiFe hydrogenase |
| 3 | I, J (A, B) | Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit | polymer | 235 | 27626.3 | 2 | UniProt (P0AAM1) Pfam (PF01292) | Escherichia coli K-12 | |
| 4 | FA, K, R, Y (R, S, T, Q) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 4 | Chemie (SF4) | |||
| 5 | GA, L, S, Z (R, S, T, Q) | FE3-S4 CLUSTER | non-polymer | 295.8 | 4 | Chemie (F3S) | |||
| 6 | AA, HA, M, T (Q, R, S, T) | Fe4S4 | non-polymer | 351.6 | 4 | Chemie (ER2) | |||
| 7 | BA, IA, N, U (J, K, L, M) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 4 | Chemie (FCO) | |||
| 8 | CA, JA, O, V (J, K, L, M) | NICKEL (II) ION | non-polymer | 58.7 | 4 | Chemie (NI) | |||
| 9 | DA, KA, P, W (J, K, L, M) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 10 | EA, LA, Q, X (J, K, L, M) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 11 | MA, NA (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 12 | OA, PA, QA, RA, SA... (S, L, T, M, Q...) | water | water | 18.0 | 702 | Chemie (HOH) |
Sequence modifications
S, T, Q, R: 1 - 327 (UniProt: P69739)
| PDB | External Database | Details |
|---|---|---|
| Gly 19 | Cys 64 | conflict |
| Arg 328 | - | expression tag |
| Ser 329 | - | expression tag |
| His 330 | - | expression tag |
| His 331 | - | expression tag |
| His 332 | - | expression tag |
| His 333 | - | expression tag |
| His 334 | - | expression tag |
| His 335 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 461332.4 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 6246.6 | |
| All* | Total formula weight | 467579.1 |
