6G90
Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | U1 snRNA,U1 snRNA,U1 snRNA,U1 snRNA,U1 snRNA | polymer | 407 | 126116.9 | 1 | GenBank (1039023797) | Saccharomyces cerevisiae | |
| 2 | B (2) | U2 snRNA | polymer | 143 | 45762.8 | 1 | GenBank (536627) | Saccharomyces cerevisiae | |
| 3 | C (A) | U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A | polymer | 298 | 33754.2 | 1 | UniProt (P32605) Pfam (PF00076) | Saccharomyces cerevisiae (Baker's yeast) | U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1 |
| 4 | D (B) | U1 small nuclear ribonucleoprotein 70 kDa homolog,U1 small nuclear ribonucleoprotein 70 kDa homolog,U1 small nuclear ribonucleoprotein 70 kDa homolog | polymer | 300 | 33445.2 | 1 | UniProt (Q00916) Pfam (PF12220) Pfam (PF00076) | Saccharomyces cerevisiae (Baker's yeast) | U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1,U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1,,U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1 |
| 5 | E (C) | U1 small nuclear ribonucleoprotein C | polymer | 231 | 27106.0 | 1 | UniProt (Q05900) Pfam (PF06220) | Saccharomyces cerevisiae (Baker's yeast) | U1C |
| 6 | F (D) | Pre-mRNA-processing factor 39 | polymer | 629 | 74834.7 | 1 | UniProt (P39682) Pfam (PF23240) Pfam (PF23241) | Saccharomyces cerevisiae (Baker's yeast) | |
| 7 | G (E) | U1 small nuclear ribonucleoprotein component PRP42 | polymer | 544 | 65222.0 | 1 | UniProt (Q03776) Pfam (PF23240) Pfam (PF23241) | Saccharomyces cerevisiae (Baker's yeast) | U1 snRNP protein PRP42,65 kDa snRNP protein,Pre-mRNA-processing factor 42 |
| 8 | H (F) | Protein NAM8 | polymer | 523 | 57010.8 | 1 | UniProt (Q00539) Pfam (PF00076) | Saccharomyces cerevisiae (Baker's yeast) | |
| 9 | I (G) | 56 kDa U1 small nuclear ribonucleoprotein component | polymer | 492 | 56575.3 | 1 | UniProt (Q03782) Pfam (PF19097) | Saccharomyces cerevisiae (Baker's yeast) | |
| 10 | J (H) | Protein LUC7,Protein LUC7,Protein LUC7 | polymer | 261 | 28504.3 | 1 | UniProt (Q07508) Pfam (PF03194) | Saccharomyces cerevisiae (Baker's yeast) | |
| 11 | K (I) | Yeast UBC4 pre-mRNA (mutant) | polymer | 38 | 12045.2 | 1 | Saccharomyces cerevisiae | ||
| 12 | L (J) | U1 small nuclear ribonucleoprotein component SNU71 | polymer | 620 | 71484.6 | 1 | UniProt (P53207) Pfam (PF24826) Pfam (PF24825) | Saccharomyces cerevisiae (Baker's yeast) | |
| 13 | M (O) | U2 snRNP component HSH155 | polymer | 971 | 110166.7 | 1 | UniProt (P49955) Pfam (PF22646) | Saccharomyces cerevisiae (Baker's yeast) | |
| 14 | N (P) | Pre-mRNA-splicing factor RSE1 | polymer | 1361 | 153956.8 | 1 | UniProt (Q04693) Pfam (PF10433) Pfam (PF23726) Pfam (PF03178) | Saccharomyces cerevisiae (Baker's yeast) | RNA splicing and ER to Golgi transport factor 1,Spliceosome-associated protein 130 |
| 15 | O (Q) | Cold sensitive U2 snRNA suppressor 1 | polymer | 435 | 50210.7 | 1 | UniProt (Q02554) Pfam (PF04037) Pfam (PF04046) | Saccharomyces cerevisiae (Baker's yeast) | |
| 16 | P (R) | Protein HSH49 | polymer | 213 | 24534.2 | 1 | UniProt (Q99181) Pfam (PF00076) | Saccharomyces cerevisiae (Baker's yeast) | |
| 17 | Q (S) | Pre-mRNA-splicing factor RDS3 | polymer | 107 | 12283.6 | 1 | UniProt (Q06835) Pfam (PF03660) | Saccharomyces cerevisiae (Baker's yeast) | Regulator of drug sensitivity 3 |
| 18 | R (T) | Pre-mRNA-splicing factor PRP9 | polymer | 530 | 63126.4 | 1 | UniProt (P19736) Pfam (PF16958) Pfam (PF16837) Pfam (PF12171) Pfam (PF11931) | Saccharomyces cerevisiae (Baker's yeast) | |
| 19 | S (U) | Pre-mRNA-splicing factor PRP11,Pre-mRNA-splicing factor PRP11,Pre-mRNA-splicing factor PRP11 | polymer | 266 | 29292.2 | 1 | UniProt (Q07350) Pfam (PF12874) Pfam (PF16835) | Saccharomyces cerevisiae (Baker's yeast) | |
| 20 | T (V) | Pre-mRNA-splicing factor PRP21 | polymer | 280 | 33111.5 | 1 | UniProt (P32524) Pfam (PF01805) Pfam (PF12230) | Saccharomyces cerevisiae (Baker's yeast) | |
| 21 | U (W) | U2 small nuclear ribonucleoprotein A' | polymer | 238 | 27232.3 | 1 | UniProt (Q08963) Pfam (PF14580) | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP A',Looks exceptionally like U2A protein 1 |
| 22 | V (X) | Unknown | polymer | 51 | 4358.4 | 1 | Saccharomyces cerevisiae | ||
| 23 | W (Y) | U2 small nuclear ribonucleoprotein B'' | polymer | 111 | 12850.9 | 1 | UniProt (P40567) Pfam (PF13893) | Saccharomyces cerevisiae (Baker's yeast) | U2 snRNP B'' |
| 24 | X (Z) | RDS3 complex subunit 10 | polymer | 85 | 10045.4 | 1 | UniProt (P0C074) Pfam (PF07189) | Saccharomyces cerevisiae (Baker's yeast) | Splicing factor 3b subunit |
| 25 | FA, Y (b, s) | Small nuclear ribonucleoprotein-associated protein B | polymer | 196 | 22427.0 | 2 | UniProt (P40018) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-B,Sm protein B,SmB |
| 26 | IA, Z (d, v) | Small nuclear ribonucleoprotein Sm D3 | polymer | 101 | 11240.1 | 2 | UniProt (P43321) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D3,snRNP core protein D3 |
| 27 | AA, JA (e, w) | Small nuclear ribonucleoprotein E | polymer | 94 | 10385.1 | 2 | UniProt (Q12330) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-E,Sm protein E,SmE |
| 28 | BA, KA (f, x) | Small nuclear ribonucleoprotein F | polymer | 86 | 9669.9 | 2 | UniProt (P54999) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-F,Sm protein F,SmF |
| 29 | CA, LA (g, y) | Small nuclear ribonucleoprotein G | polymer | 77 | 8490.8 | 2 | UniProt (P40204) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | snRNP-G,Sm protein G,SmG |
| 30 | DA, GA (h, t) | Small nuclear ribonucleoprotein Sm D1 | polymer | 146 | 16296.8 | 2 | UniProt (Q02260) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D1,snRNP core protein D1 |
| 31 | EA, HA (i, u) | Small nuclear ribonucleoprotein Sm D2 | polymer | 110 | 12876.1 | 2 | UniProt (Q06217) Pfam (PF01423) | Saccharomyces cerevisiae (Baker's yeast) | Sm-D2,snRNP core protein D2 |
| 32 | MA, NA, OA, PA, QA... (C, H, S, T, U) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) |
Sequence modifications
1: 1 - 6 (PDB: 6G90)
1: 7 - 20 (PDB: 6G90)
1: 21 - 325 (GenBank: 1039023797)
1: 378 - 440 (PDB: 6G90)
1: 516 - 568 (PDB: 6G90)
A: 1 - 118 (UniProt: P32605)
A: 119 - 123 (PDB: 6G90)
A: 124 - 133 (PDB: 6G90)
A: 134 - 148 (PDB: 6G90)
A: 149 - 298 (UniProt: P32605)
B: 1 - 57 (UniProt: Q00916)
B: 58 - 88 (PDB: 6G90)
B: 89 - 300 (UniProt: Q00916)
H: 1 - 261 (UniProt: Q07508)
H: 91 - 187 (PDB: 6G90)
H: 187 - 261 (PDB: 6G90)
U: 1 - 114 (UniProt: Q07350)
U: 115 - 136 (PDB: 6G90)
U: 137 - 266 (UniProt: Q07350)
1: 7 - 20 (PDB: 6G90)
1: 21 - 325 (GenBank: 1039023797)
1: 378 - 440 (PDB: 6G90)
1: 516 - 568 (PDB: 6G90)
A: 1 - 118 (UniProt: P32605)
A: 119 - 123 (PDB: 6G90)
A: 124 - 133 (PDB: 6G90)
A: 134 - 148 (PDB: 6G90)
A: 149 - 298 (UniProt: P32605)
B: 1 - 57 (UniProt: Q00916)
B: 58 - 88 (PDB: 6G90)
B: 89 - 300 (UniProt: Q00916)
H: 1 - 261 (UniProt: Q07508)
| PDB | External Database | Details |
|---|---|---|
| Val 83 | Glu 83 | conflict |
| Glu 84 | Val 84 | conflict |
H: 187 - 261 (PDB: 6G90)
U: 1 - 114 (UniProt: Q07350)
U: 115 - 136 (PDB: 6G90)
U: 137 - 266 (UniProt: Q07350)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 38 |
| Total formula weight | 1345802.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 588.7 | |
| All* | Total formula weight | 1346391.4 |
