6G90

Prespliceosome structure provides insight into spliceosome assembly and regulation (map A2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	U1 snRNA,U1 snRNA,U1 snRNA,U1 snRNA,U1 snRNA	polymer	407	126116.9	1	GenBank (1039023797)	Saccharomyces cerevisiae
2	2	U2 snRNA	polymer	143	45762.8	1	GenBank (536627)	Saccharomyces cerevisiae
3	A	U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A,U1 small nuclear ribonucleoprotein A	polymer	298	33754.2	1	UniProt (P32605) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1,U1A,Mutant U1 die protein 1
4	B	U1 small nuclear ribonucleoprotein 70 kDa homolog,U1 small nuclear ribonucleoprotein 70 kDa homolog,U1 small nuclear ribonucleoprotein 70 kDa homolog	polymer	300	33445.2	1	UniProt (Q00916) Pfam (PF12220) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1,U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1,,U1-70K,U1 small nuclear ribonucleoprotein SNP1,U1 snRNP protein SNP1
5	C	U1 small nuclear ribonucleoprotein C	polymer	231	27106.0	1	UniProt (Q05900) Pfam (PF06220) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U1C
6	D	Pre-mRNA-processing factor 39	polymer	629	74834.7	1	UniProt (P39682) In PDB	Saccharomyces cerevisiae (Baker's yeast)
7	E	U1 small nuclear ribonucleoprotein component PRP42	polymer	544	65222.0	1	UniProt (Q03776) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U1 snRNP protein PRP42,65 kDa snRNP protein,Pre-mRNA-processing factor 42
8	F	Protein NAM8	polymer	523	57010.8	1	UniProt (Q00539) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)
9	G	56 kDa U1 small nuclear ribonucleoprotein component	polymer	492	56575.3	1	UniProt (Q03782) Pfam (PF19097) In PDB	Saccharomyces cerevisiae (Baker's yeast)
10	H	Protein LUC7,Protein LUC7,Protein LUC7	polymer	261	28504.3	1	UniProt (Q07508) Pfam (PF03194) In PDB	Saccharomyces cerevisiae (Baker's yeast)
11	I	Yeast UBC4 pre-mRNA (mutant)	polymer	38	12045.2	1		Saccharomyces cerevisiae
12	J	U1 small nuclear ribonucleoprotein component SNU71	polymer	620	71484.6	1	UniProt (P53207) In PDB	Saccharomyces cerevisiae (Baker's yeast)
13	O	U2 snRNP component HSH155	polymer	971	110166.7	1	UniProt (P49955) Pfam (PF22646) In PDB	Saccharomyces cerevisiae (Baker's yeast)
14	P	Pre-mRNA-splicing factor RSE1	polymer	1361	153956.8	1	UniProt (Q04693) Pfam (PF10433) Pfam (PF03178) In PDB	Saccharomyces cerevisiae (Baker's yeast)	RNA splicing and ER to Golgi transport factor 1,Spliceosome-associated protein 130
15	Q	Cold sensitive U2 snRNA suppressor 1	polymer	435	50210.7	1	UniProt (Q02554) Pfam (PF04037) Pfam (PF04046) In PDB	Saccharomyces cerevisiae (Baker's yeast)
16	R	Protein HSH49	polymer	213	24534.2	1	UniProt (Q99181) Pfam (PF00076) In PDB	Saccharomyces cerevisiae (Baker's yeast)
17	S	Pre-mRNA-splicing factor RDS3	polymer	107	12283.6	1	UniProt (Q06835) Pfam (PF03660) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Regulator of drug sensitivity 3
18	T	Pre-mRNA-splicing factor PRP9	polymer	530	63126.4	1	UniProt (P19736) Pfam (PF16958) Pfam (PF16837) Pfam (PF12171) Pfam (PF11931) In PDB	Saccharomyces cerevisiae (Baker's yeast)
19	U	Pre-mRNA-splicing factor PRP11,Pre-mRNA-splicing factor PRP11,Pre-mRNA-splicing factor PRP11	polymer	266	29292.2	1	UniProt (Q07350) Pfam (PF12874) Pfam (PF16835) In PDB	Saccharomyces cerevisiae (Baker's yeast)
20	V	Pre-mRNA-splicing factor PRP21	polymer	280	33111.5	1	UniProt (P32524) Pfam (PF01805) Pfam (PF12230) In PDB	Saccharomyces cerevisiae (Baker's yeast)
21	W	U2 small nuclear ribonucleoprotein A'	polymer	238	27232.3	1	UniProt (Q08963) Pfam (PF14580) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U2 snRNP A',Looks exceptionally like U2A protein 1
22	X	Unknown	polymer	51	4358.4	1		Saccharomyces cerevisiae
23	Y	U2 small nuclear ribonucleoprotein B''	polymer	111	12850.9	1	UniProt (P40567) Pfam (PF13893) In PDB	Saccharomyces cerevisiae (Baker's yeast)	U2 snRNP B''
24	Z	RDS3 complex subunit 10	polymer	85	10045.4	1	UniProt (P0C074) Pfam (PF07189) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Splicing factor 3b subunit
25	b, s	Small nuclear ribonucleoprotein-associated protein B	polymer	196	22427.0	2	UniProt (P40018) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-B,Sm protein B,SmB
26	d, v	Small nuclear ribonucleoprotein Sm D3	polymer	101	11240.1	2	UniProt (P43321) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D3,snRNP core protein D3
27	e, w	Small nuclear ribonucleoprotein E	polymer	94	10385.1	2	UniProt (Q12330) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-E,Sm protein E,SmE
28	f, x	Small nuclear ribonucleoprotein F	polymer	86	9669.9	2	UniProt (P54999) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-F,Sm protein F,SmF
29	g, y	Small nuclear ribonucleoprotein G	polymer	77	8490.8	2	UniProt (P40204) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	snRNP-G,Sm protein G,SmG
30	h, t	Small nuclear ribonucleoprotein Sm D1	polymer	146	16296.8	2	UniProt (Q02260) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D1,snRNP core protein D1
31	i, u	Small nuclear ribonucleoprotein Sm D2	polymer	110	12876.1	2	UniProt (Q06217) Pfam (PF01423) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Sm-D2,snRNP core protein D2
32	C, H, S, T, U	ZINC ION	non-polymer		65.4	9	Chemie (ZN)

Sequence modifications

1: 1 - 6 (PDB: 6G90)
1: 7 - 20 (PDB: 6G90)
1: 21 - 325 (GenBank: 1039023797)
1: 378 - 440 (PDB: 6G90)
1: 516 - 568 (PDB: 6G90)
A: 1 - 118 (UniProt: P32605)
A: 119 - 123 (PDB: 6G90)
A: 124 - 133 (PDB: 6G90)
A: 134 - 148 (PDB: 6G90)
A: 149 - 298 (UniProt: P32605)
B: 1 - 57 (UniProt: Q00916)
B: 58 - 88 (PDB: 6G90)
B: 89 - 300 (UniProt: Q00916)
H: 1 - 261 (UniProt: Q07508)

PDB	External Database	Details
Val 83	Glu 83	conflict
Glu 84	Val 84	conflict

H: 91 - 187 (PDB: 6G90)
H: 187 - 261 (PDB: 6G90)
U: 1 - 114 (UniProt: Q07350)
U: 115 - 136 (PDB: 6G90)
U: 137 - 266 (UniProt: Q07350)

Contents of the asymmetric unit

Polymers	Number of chains	38
	Total formula weight	1345802.8
Non-Polymers*	Number of molecules	9
	Total formula weight	588.7
All*	Total formula weight	1346391.4

*Water molecules are not included.