6G26

The crystal structure of the Burkholderia pseudomallei HicAB complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	HicB	polymer	142	15762.7	4	UniProt (Q63NA5) Pfam (PF15919)	Burkholderia pseudomallei K96243
2	E, F, G, H (E, F, G, H)	HicA	polymer	64	7067.3	4	UniProt (Q63NA6) Pfam (PF07927)	Burkholderia pseudomallei K96243
3	I, J, R, W, Z (A, B, C, D)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	AA, BA, CA, DA, FA... (D, H, A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	18	Chemie (EDO)
5	EA (D)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	GA, HA, IA, JA, KA... (A, B, C, D, E...)	water	water		18.0	269	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 138 (UniProt: Q63NA5)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 136	Val 135	conflict
His 137	Arg 136	conflict
His 139	-	expression tag
His 140	-	expression tag
His 141	-	expression tag
His 142	-	expression tag

E, F, G, H: 2 - 59 (UniProt: Q63NA6)

PDB	External Database	Details
Gly -4	-	expression tag
Ile -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Phe 0	-	expression tag
Thr 1	-	expression tag
Ala 24	His 24	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	91320.1
Non-Polymers*	Number of molecules	24
	Total formula weight	1747.7
All*	Total formula weight	93067.8

*Water molecules are not included.