6FZA

Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant A187F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Lipase	polymer	391	43922.0	1	UniProt (Q93A71) In PDB	Geobacillus stearothermophilus
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	205	Chemie (HOH)

Sequence modifications

A: 5 - 389 (UniProt: Q93A71)

PDB	External Database	Details
Phe 187	Ala 216	engineered mutation
Ala 323	Thr 352	conflict
His 390	-	expression tag
His 391	-	expression tag
His 392	-	expression tag
His 393	-	expression tag
His 394	-	expression tag
His 395	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43922.0
Non-Polymers*	Number of molecules	2
	Total formula weight	105.5
All*	Total formula weight	44027.5

*Water molecules are not included.