6FWW

GFP/KKK. A redesigned GFP with improved solubility

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	266	29851.6	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	B (A)	water	water		18.0	334	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -14	-	initiating methionine
Ala -13	-	expression tag
Ser -12	-	expression tag
Met -11	-	expression tag
Thr -10	-	expression tag
Gly -9	-	expression tag
Gly -8	-	expression tag
Gln -7	-	expression tag
Gln -6	-	expression tag
Met -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Asp -2	-	expression tag
Pro -1	-	expression tag
Asn 0	-	expression tag
Ser 1	-	expression tag
Lys 11	Val 11	engineered mutation
Lys 39	Tyr 39	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 65	Ser 65	chromophore
Cro 65	Tyr 66	chromophore
Cro 65	Gly 67	chromophore
Arg 80	Gln 80	conflict
Ser 99	Phe 99	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Lys 221	Leu 221	engineered mutation
Lys 239	-	expression tag
Leu 240	-	expression tag
Ala 241	-	expression tag
Ala 242	-	expression tag
Ala 243	-	expression tag
Leu 244	-	expression tag
Glu 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29851.6
All*	Total formula weight	29851.6

*Water molecules are not included.