6FWO
Structure of an E336Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with alpha-Glc-1,3-1,2-anhydro-mannose hydrolyzed by enzyme
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Glycosyl hydrolase family 71 | polymer | 385 | 43932.6 | 1 | UniProt (D6D1V7) Pfam (PF16317) In PDB | Bacteroides xylanisolvens XB1A | |
2 | A | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
3 | A | (1~{S},2~{R},3~{S},4~{R},5~{R})-5-(hydroxymethyl)cyclohexane-1,2,3,4-tetrol | non-polymer | 178.2 | 1 | Chemie (M96) | |||
4 | A | alpha-D-glucopyranose | non-polymer | 180.2 | 1 | Chemie (GLC) | |||
5 | water | water | 18.0 | 275 | Chemie (HOH) |
Sequence modifications
A: 17 - 380 (UniProt: D6D1V7)
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
His 2 | - | expression tag |
His 3 | - | expression tag |
His 4 | - | expression tag |
His 5 | - | expression tag |
Ser 6 | - | expression tag |
Ser 7 | - | expression tag |
Gly 8 | - | expression tag |
Leu 9 | - | expression tag |
Val 10 | - | expression tag |
Pro 11 | - | expression tag |
Arg 12 | - | expression tag |
Gly 13 | - | expression tag |
Ser 14 | - | expression tag |
His 15 | - | expression tag |
Met 16 | - | expression tag |
Gln 336 | Glu 336 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 43932.6 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 476.4 | |
All* | Total formula weight | 44409.0 |