6FV4

The structure of N-acetyl-D-glucosamine-6-phosphate deacetylase D267A mutant from Mycobacterium smegmatis in complex with N-acetyl-D-glucosamine-6-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	N-acetylglucosamine-6-phosphate deacetylase	polymer	401	41272.7	2	UniProt (A0QU89) Pfam (PF01979)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
2	C, H (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	D (A)	2-acetamido-2-deoxy-6-O-phosphono-alpha-D-glucopyranose	non-polymer		301.2	1	Chemie (16G)
4	E, F, I, J (A, B)	CADMIUM ION	non-polymer		112.4	4	Chemie (CD)
5	G, K (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	L (B)	2,3-DIHYDROXY-1,4-DITHIOBUTANE	non-polymer		154.3	1	Chemie (DTT)
7	M, N (A, B)	water	water		18.0	527	Chemie (HOH)

Sequence modifications

A, B: 1 - 385 (UniProt: A0QU89)

PDB	External Database	Details
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 267	Asp 267	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82545.5
Non-Polymers*	Number of molecules	10
	Total formula weight	1106.8
All*	Total formula weight	83652.3

*Water molecules are not included.