6FRE
Crystal structure of G-1F/H73A mutant of Ssp DnaB Mini-Intein variant M86
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Replicative DNA helicase,Replicative DNA helicase | polymer | 169 | 18909.8 | 1 | UniProt (Q55418) Pfam (PF14890) Pfam (PF03796) | Synechocystis sp. (strain PCC 6803 / Kazusa) | |
| 2 | B (A) | water | water | 18.0 | 247 | Chemie (HOH) |
Sequence modifications
A: -5 - 106 (UniProt: Q55418)
A: 112 - 159 (UniProt: Q55418)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | initiating methionine |
| Phe -1 | Gly 380 | engineered mutation |
| Ala 1 | Cys 381 | engineered mutation |
| Pro 18 | Ser 398 | engineered mutation |
| Gly 24 | Asp 404 | engineered mutation |
| Thr 58 | Ile 438 | engineered mutation |
| Ala 73 | His 453 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala 107 | - | linker |
| Pro 108 | - | linker |
| Glu 109 | - | linker |
| Ile 110 | - | linker |
| Glu 111 | - | linker |
| Pro 114 | Ser 769 | engineered mutation |
| Pro 122 | Ser 777 | engineered mutation |
| Leu 142 | Pro 797 | engineered mutation |
| Arg 143 | His 798 | engineered mutation |
| Ala 154 | Asn 809 | engineered mutation |
| Lys 160 | - | expression tag |
| Leu 161 | - | expression tag |
| Gly 162 | - | expression tag |
| Gly 163 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 18909.8 | |
| All* | Total formula weight | 18909.8 |
