6FRE

Crystal structure of G-1F/H73A mutant of Ssp DnaB Mini-Intein variant M86

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Replicative DNA helicase,Replicative DNA helicase	polymer	169	18909.8	1	UniProt (Q55418) Pfam (PF14890) Pfam (PF03796) In PDB	Synechocystis sp. (strain PCC 6803 / Kazusa)
2		water	water		18.0	247	Chemie (HOH)

Sequence modifications

A: -5 - 106 (UniProt: Q55418)

PDB	External Database	Details
Met -6	-	initiating methionine
Phe -1	Gly 380	engineered mutation
Ala 1	Cys 381	engineered mutation
Pro 18	Ser 398	engineered mutation
Gly 24	Asp 404	engineered mutation
Thr 58	Ile 438	engineered mutation
Ala 73	His 453	engineered mutation

A: 112 - 159 (UniProt: Q55418)

PDB	External Database	Details
Ala 107	-	linker
Pro 108	-	linker
Glu 109	-	linker
Ile 110	-	linker
Glu 111	-	linker
Pro 114	Ser 769	engineered mutation
Pro 122	Ser 777	engineered mutation
Leu 142	Pro 797	engineered mutation
Arg 143	His 798	engineered mutation
Ala 154	Asn 809	engineered mutation
Lys 160	-	expression tag
Leu 161	-	expression tag
Gly 162	-	expression tag
Gly 163	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18909.8
All*	Total formula weight	18909.8

*Water molecules are not included.