6FML

CryoEM Structure INO80core Nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	RuvB-like helicase	polymer	462	50451.8	3	UniProt (G0RYI5) Pfam (PF06068) Pfam (PF17856)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
2	D, E, F (D, E, F)	RuvB-like helicase	polymer	488	53212.7	3	UniProt (G0RYC2) Pfam (PF06068) Pfam (PF17856)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
3	G (G)	Ino80	polymer	1856	210443.3	1		Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
4	H (H)	les2	polymer	491	52018.5	1	UniProt (G0RY01) Pfam (PF04795)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
5	I (I)	Ies6	polymer	219	23127.5	1	UniProt (G0S590) Pfam (PF08265)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
6	J (J)	Actin related protein 5	polymer	770	85996.5	1	UniProt (G0S589) Pfam (PF00022)	Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
7	K (K)	Nucleosomal DNA Strand 1	polymer	196	60652.6	1		synthetic construct
8	L (L)	Nucleosomal DNA Strand 2	polymer	196	60376.4	1		synthetic construct
9	M, Q (M, Q)	Histone H3.2	polymer	135	15289.9	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/m,Histone H3/o
10	N, R (N, R)	Histone H4	polymer	102	11263.2	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
11	O, S (O, S)	Histone H2A type 1	polymer	129	13990.3	2	UniProt (P0C0S9) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P0C0S8)	Homo sapiens (Human)
12	P, T (P, T)	Histone H2B type 1-C/E/F/G/I	polymer	125	13806.0	2	UniProt (P62807) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l
13	U, V, W, X, Y... (A, B, C, D, E...)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	6	Chemie (ADP)
14	AA (J)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)

Sequence modifications

H: 2 - 383 (UniProt: G0RY01)
H: 384 - 414 (PDB: 6FML)
H: 415 - 492 (UniProt: G0RY01)
J: 1 - 470 (UniProt: G0S589)
J: 471 - 498 (PDB: 6FML)
J: 498 - 498 (PDB: 6FML)
J: 498 - 519 (PDB: 6FML)
J: 519 - 520 (UniProt: G0S589)
J: 520 - 584 (PDB: 6FML)
J: 586 - 769 (UniProt: G0S589)

Contents of the asymmetric unit

Polymers	Number of chains	20
	Total formula weight	912307.7
Non-Polymers*	Number of molecules	7
	Total formula weight	3070.4
All*	Total formula weight	915378.1

*Water molecules are not included.