6FHV

Crystal structure of Penicillium oxalicum Glucoamylase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glucoamylase	polymer	594	65448.8	1	UniProt (S7ZIW0) Pfam (PF00723) Pfam (PF00686)	Penicillium oxalicum (strain 114-2 / CGMCC 5302) (Penicillium decumbens)	1,4-alpha-D-glucan glucohydrolase,Glucan 1,4-alpha-glucosidase
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C (C)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
4	D (A)	2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		282.3	1	Chemie (B3P)
5	E (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
6	F (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	G (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
8	H (A)	water	water		18.0	257	Chemie (HOH)

Sequence modifications

A: 23 - 615 (UniProt: S7ZIW0)

PDB	External Database	Details
Asn 74	Asp 74	conflict
Val 100	Lys 100	conflict
Arg 113	Gln 113	conflict
Ser 400	Pro 400	conflict
Tyr 406	Phe 406	conflict
Lys 408	Ser 408	conflict
Ser 415	Ala 415	conflict
Ala 444	Thr 444	conflict
Val 508	Ile 508	conflict
Phe 616	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	65448.8
Branched	Number of molecules	2
	Total formula weight	1010.9
Non-Polymers*	Number of molecules	4
	Total formula weight	803.9
All*	Total formula weight	67263.6

*Water molecules are not included.