6FH8

E. coli surface display of streptavidin for directed evolution of an allylic deallocase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Streptavidin	polymer	159	16556.0	1	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A	biotinylated ruthenium cyclopentadienide	non-polymer		659.7	1	Chemie (JCT)
3		water	water		18.0	79	Chemie (HOH)

Sequence modifications

A: 14 - 159 (UniProt: P22629)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Met 112	Ser 136	engineered mutation
Ala 121	Lys 145	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16556.0
Non-Polymers*	Number of molecules	1
	Total formula weight	659.7
All*	Total formula weight	17215.7

*Water molecules are not included.