6FG0

Crystal structure of R. ruber ADH-A, mutant Y54G, F43T, L119Y, F282W

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Alcohol dehydrogenase	polymer	352	36036.9	4	UniProt (A0A1Q8I6M1)	Rhodococcus sp. M8
2	E, F, H, I, K... (A, B, C, D)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
3	G, J, M, P (A, B, C, D)	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	4	Chemie (NAD)
4	Q, R, S, T (A, B, C, D)	water	water		18.0	1381	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 345 (UniProt: A0A1Q8I6M1)

PDB	External Database	Details
Thr 43	Phe 43	engineered mutation
Gly 54	Tyr 54	engineered mutation
Tyr 119	Leu 119	engineered mutation
Trp 282	Phe 282	engineered mutation
Thr 346	-	expression tag
Ser 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag
His 350	-	expression tag
His 351	-	expression tag
His 352	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	144147.5
Non-Polymers*	Number of molecules	12
	Total formula weight	3177.0
All*	Total formula weight	147324.5

*Water molecules are not included.