6FBU
Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli (E2Q) in complex with AP-site containing DNA substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Endonuclease 8 | polymer | 262 | 29814.0 | 1 | UniProt (P50465) Pfam (PF01149) Pfam (PF06831) Pfam (PF06827) In PDB | Escherichia coli | DNA glycosylase/AP lyase Nei,DNA-(apurinic or apyrimidinic site) lyase Nei,Endonuclease VIII |
2 | B | DNA (5'-D(P*GP*GP*CP*TP*TP*CP*AP*TP*CP*CP*TP*G)-3') | polymer | 13 | 3958.6 | 1 | synthetic construct | ||
3 | C | DNA (5'-D(P*CP*CP*AP*GP*GP*AP*(PED)P*GP*AP*AP*GP*CP*C)-3') | polymer | 13 | 3863.5 | 1 | synthetic construct | ||
4 | A | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
5 | A | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
6 | A | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
7 | water | water | 18.0 | 205 | Chemie (HOH) |
Sequence modifications
A: 1 - 262 (UniProt: P50465)
PDB | External Database | Details |
---|---|---|
Gln 2 | Glu 3 | engineered mutation |
Thr 34 | Pro 35 | engineered mutation |
Arg 112 | Thr 113 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 37636.1 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 412.6 | |
All* | Total formula weight | 38048.8 |