6FBU
Crystal structure of the DNA repair enzyme endonuclease-VIII (Nei) from E. coli (E2Q) in complex with AP-site containing DNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Endonuclease 8 | polymer | 262 | 29814.0 | 1 | UniProt (P50465) Pfam (PF01149) Pfam (PF06831) Pfam (PF06827) | Escherichia coli | DNA glycosylase/AP lyase Nei,DNA-(apurinic or apyrimidinic site) lyase Nei,Endonuclease VIII |
| 2 | B (B) | DNA (5'-D(P*GP*GP*CP*TP*TP*CP*AP*TP*CP*CP*TP*G)-3') | polymer | 13 | 3958.6 | 1 | synthetic construct | ||
| 3 | C (C) | DNA (5'-D(P*CP*CP*AP*GP*GP*AP*(PED)P*GP*AP*AP*GP*CP*C)-3') | polymer | 13 | 3863.5 | 1 | synthetic construct | ||
| 4 | D, E, F (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 5 | G (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 6 | H (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 7 | I, J, K (A, B, C) | water | water | 18.0 | 205 | Chemie (HOH) |
Sequence modifications
A: 1 - 262 (UniProt: P50465)
| PDB | External Database | Details |
|---|---|---|
| Gln 2 | Glu 3 | engineered mutation |
| Thr 34 | Pro 35 | engineered mutation |
| Arg 112 | Thr 113 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 37636.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 412.6 | |
| All* | Total formula weight | 38048.8 |
