6FB8
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Magnesium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (D) | DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP*GP*CP*AP*GP*TP*TP*TP*TP*GP*A)-3') | polymer | 24 | 7368.8 | 1 | synthetic construct | ||
| 2 | B (C) | DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*TP*GP*CP*GP*TP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3') | polymer | 24 | 7368.8 | 1 | synthetic construct | ||
| 3 | C, D (A, B) | DNA endonuclease I-CreI | polymer | 154 | 17657.3 | 2 | UniProt (P05725) Pfam (PF00961) | Chlamydomonas reinhardtii (Chlamydomonas smithii) | 23S rRNA intron protein |
| 4 | E, F, I, L (D, A, B) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 5 | G, H, J, K (A, B) | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 4 | Chemie (PGO) | |||
| 6 | M, N, O, P (D, C, A, B) | water | water | 18.0 | 86 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 153 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Asp 75 | engineered mutation |
| Ala 154 | - | expression tag |
| Ala 155 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 50052.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 401.6 | |
| All* | Total formula weight | 50453.7 |
