6FB7
Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA endonuclease I-CreI | polymer | 152 | 17515.1 | 2 | UniProt (P05725) Pfam (PF00961) | Chlamydomonas reinhardtii (Chlamydomonas smithii) | 23S rRNA intron protein |
| 2 | C, E (D, F) | DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*C)-3') | polymer | 14 | 4248.8 | 2 | synthetic construct | ||
| 3 | D, F (E, G) | DNA (5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3') | polymer | 10 | 3075.0 | 2 | synthetic construct | ||
| 4 | G, H, I (A) | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
| 5 | J, K, L, M (A, B, E, F) | water | water | 18.0 | 22 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 153 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Asp 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 49677.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 164.8 | |
| All* | Total formula weight | 49842.7 |
