6FB5
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Magnesium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | I-CreI monomer A | polymer | 153 | 17528.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) In PDB | Chlamydomonas reinhardtii | |
| 2 | B | I-CreI monomer B | polymer | 154 | 17583.2 | 1 | Pfam (PF00961) UniProt (by SIFTS) (P05725) In PDB | Chlamydomonas reinhardtii | |
| 3 | D | DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*CP*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3') | polymer | 24 | 7378.8 | 1 | synthetic construct | ||
| 4 | F | DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*GP*AP*CP*AP*AP*GP*TP*CP*TP*GP*A)-3') | polymer | 24 | 7360.8 | 1 | synthetic construct | ||
| 5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 6 | B | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 7 | D | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 1 | Chemie (PGO) | |||
| 8 | water | water | 18.0 | 121 | Chemie (HOH) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49850.9 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 232.4 | |
| All* | Total formula weight | 50083.3 |
