6F8N

Key residues affecting transglycosylation activity in family 18 chitinases - Insights into donor and acceptor subsites

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Glycoside hydrolase family 18	polymer	414	45757.5	2	UniProt (A8GFD6) Pfam (PF00704)	Serratia proteamaculans (strain 568)
2	C, D (C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	E, F, H, I (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
4	G, J (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	K, L (A, B)	water	water		18.0	995	Chemie (HOH)

Sequence modifications

A, B: 17 - 420 (UniProt: A8GFD6)

PDB	External Database	Details
Met 13	-	initiating methionine
Gly 14	-	expression tag
Ala 15	-	expression tag
Gly 16	-	expression tag
Gln 153	Glu 159	engineered mutation
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91515.0
Branched	Number of molecules	2
	Total formula weight	848.8
Non-Polymers*	Number of molecules	6
	Total formula weight	319.2
All*	Total formula weight	92683.0

*Water molecules are not included.