6F6C

R2-like ligand-binding oxidase V72A mutant with aerobically reconstituted Mn/Fe cofactor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Ribonucleotide reductase small subunit	polymer	316	36934.8	2	UniProt (Q5KW80) Pfam (PF00268) In PDB	Geobacillus kaustophilus (strain HTA426)
2	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	A, B	MANGANESE (III) ION	non-polymer		54.9	2	Chemie (MN3)
4	A, B	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
5	A, B	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
6		water	water		18.0	152	Chemie (HOH)

Sequence modifications

A, B: 1 - 302 (UniProt: Q5KW80)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Ala 72	Val 72	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73869.5
Non-Polymers*	Number of molecules	8
	Total formula weight	844.3
All*	Total formula weight	74713.8

*Water molecules are not included.