6F5E

Crystal structure of DARPin-DARPin rigid fusion, variant DD_D12_10_47 in complex JNK1a1 and JIP1 peptide

Replaces:  5LEN

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DD_D12_10_47	polymer	325	35112.6	1		synthetic construct
2	B (B)	Mitogen-activated protein kinase 8	polymer	373	43154.9	1	UniProt (P45983) Pfam (PF00069)	Homo sapiens (Human)	MAPK 8,JNK-46,Stress-activated protein kinase 1c,SAPK1c,Stress-activated protein kinase JNK1,c-Jun N-terminal kinase 1
3	C (C)	C-Jun-amino-terminal kinase-interacting protein 1	polymer	11	1345.6	1	UniProt (Q9WVI9)	Mus musculus	JNK-interacting protein 1,Islet-brain-1,IB-1,JNK MAP kinase scaffold protein 1,Mitogen-activated protein kinase 8-interacting protein 1

Sequence modifications

B: 2 - 363 (UniProt: P45983)

PDB	External Database	Details
Met -9	-	initiating methionine
Arg -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	79613.1
All*	Total formula weight	79613.1

*Water molecules are not included.