6F56

Mutant of Human N-myristoyltransferase with bound myristoyl-CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glycylpeptide N-tetradecanoyltransferase 1	polymer	410	47596.8	4	UniProt (P30419) Pfam (PF01233) Pfam (PF02799)	Homo sapiens (Human)	Myristoyl-CoA:protein N-myristoyltransferase 1,Type I N-myristoyltransferase,Peptide N-myristoyltransferase 1
2	E, I, M, Q (A, B, C, D)	TETRADECANOYL-COA	non-polymer		977.9	4	Chemie (MYA)
3	F, J, N, R (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, H, K, L, O... (A, B, C, D)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
5	V, W, X, Y (A, B, C, D)	water	water		18.0	463	Chemie (HOH)

Sequence modifications

A, B, C, D: 109 - 496 (UniProt: P30419)

PDB	External Database	Details
Met 87	-	initiating methionine
Gly 88	-	expression tag
Ser 89	-	expression tag
Ser 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag
His 94	-	expression tag
His 95	-	expression tag
His 96	-	expression tag
Ser 97	-	expression tag
Ser 98	-	expression tag
Gly 99	-	expression tag
Leu 100	-	expression tag
Glu 101	-	expression tag
Val 102	-	expression tag
Leu 103	-	expression tag
Phe 104	-	expression tag
Gln 105	-	expression tag
Gly 106	-	expression tag
Pro 107	-	expression tag
His 108	-	expression tag
Gln 295	Arg 215	engineered mutation
Phe 297	Trp 217	engineered mutation
Met 452	Ala 372	engineered mutation
Val 453	Leu 373	engineered mutation
Val 462	Leu 382	engineered mutation
His 473	Asn 393	engineered mutation
Met 495	Leu 415	engineered mutation
Leu 496	Gln 416	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	190387.0
Non-Polymers*	Number of molecules	17
	Total formula weight	4837.6
All*	Total formula weight	195224.7

*Water molecules are not included.