6EZN
Cryo-EM structure of the yeast oligosaccharyltransferase (OST) complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 | polymer | 476 | 54116.5 | 1 | UniProt (P41543) Pfam (PF04597) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase 64 kDa subunit,Oligosaccharyl transferase subunit OST1,Oligosaccharyl transferase subunit alpha |
| 2 | B (B) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2 | polymer | 130 | 14712.5 | 1 | UniProt (P46964) Pfam (PF02109) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit OST2,Oligosaccharyl transferase 16 kDa subunit,Oligosaccharyl transferase subunit epsilon |
| 3 | C (C) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 | polymer | 350 | 39518.2 | 1 | UniProt (P48439) Pfam (PF04756) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase 34 kDa subunit,Oligosaccharyl transferase subunit OST3,Oligosaccharyl transferase subunit gamma |
| 4 | D (D) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST4 | polymer | 36 | 3986.7 | 1 | UniProt (Q99380) Pfam (PF10215) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit OST4,Oligosaccharyl transferase 4 kDa subunit,OTase 4 kDa subunit |
| 5 | E (E) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST5 | polymer | 86 | 9525.1 | 1 | UniProt (Q92316) Pfam (PF05251) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit OST5,Oligosaccharyl transferase 9.5 kDa subunit,Oligosaccharyl transferase subunit zeta |
| 6 | F (F) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3 | polymer | 718 | 81604.5 | 1 | UniProt (P39007) Pfam (PF02516) Pfam (PF21436) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit STT3 |
| 7 | G (G) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit WBP1 | polymer | 430 | 49444.4 | 1 | UniProt (P33767) Pfam (PF03345) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit WBP1,Oligosaccharyl transferase subunit beta |
| 8 | H (H) | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit SWP1 | polymer | 286 | 31682.8 | 1 | UniProt (Q02795) Pfam (PF05817) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Oligosaccharyl transferase subunit SWP1,Oligosaccharyl transferase subunit delta |
| 9 | I (I) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 10 | J, L (J, L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 2 | In PDB GlyTouCan (G42666HT) | |||
| 11 | K (K) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G83582BK) | |||
| 12 | M, N, O, R (A, E, F, H) | 1-PALMITOYL-2-LINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 758.1 | 4 | Chemie (CPL) | |||
| 13 | P (F) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
| 14 | Q (G) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 284590.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2832.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 3987.5 | |
| All* | Total formula weight | 291410.8 |
