6EYD

Structure of Mycobacterium smegmatis RNA polymerase Sigma-A holoenzyme

Replaces:  6FED

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	350	37959.4	2	UniProt (A0QSL8) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1178	129456.9	1	UniProt (P60281) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1325	147786.0	1	UniProt (A0QS66) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	107	11512.7	1	UniProt (A0QWT1) Pfam (PF01192)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	466	51573.6	1	UniProt (A0QW02) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
6	G, H (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	I (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

C: 2 - 1169 (UniProt: P60281)

PDB	External Database	Details
Val 1	-	expression tag
Leu 1170	-	expression tag
Ala 1171	-	expression tag
Arg 1172	-	expression tag
His 1173	-	expression tag
Gly 1174	-	expression tag
Gly 1175	-	expression tag
Ser 1176	-	expression tag
Gly 1177	-	expression tag
Ala 1178	-	expression tag

D: 2 - 1317 (UniProt: A0QS66)

PDB	External Database	Details
Val 1	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag
His 1325	-	expression tag

E: 2 - 107 (UniProt: A0QWT1)

PDB	External Database	Details
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	416248.0
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	416403.1

*Water molecules are not included.