6EXV
Structure of mammalian RNA polymerase II elongation complex inhibited by Alpha-amanitin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1 | polymer | 1970 | 217450.1 | 1 | UniProt (I3LJR4) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB | Sus scrofa (Pig) | |
| 2 | B | DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB2 | polymer | 1167 | 133201.6 | 1 | UniProt (I3LGP4) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB | Sus scrofa (Pig) | |
| 3 | C | DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) In PDB | Sus scrofa (Pig) | |
| 4 | D | DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3 | polymer | 142 | 16331.3 | 1 | UniProt (A0A287ADR4) Pfam (PF03874) In PDB | Sus scrofa (Pig) | |
| 5 | E | DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1 | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) In PDB | Sus scrofa (Pig) | |
| 6 | F | DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1 | polymer | 127 | 14477.0 | 1 | UniProt (F1SKN8) Pfam (PF01192) UniProt (by SIFTS) (A0A4X1VEK9) In PDB | Sus scrofa (Pig) | |
| 7 | G | DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7 | polymer | 172 | 19314.3 | 1 | UniProt (I3LJZ9) In PDB | Sus scrofa (Pig) | |
| 8 | H | DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) In PDB | Sus scrofa (Pig) | |
| 9 | I | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) In PDB | Sus scrofa (Pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | Sus Scrofa (Pig) | RNA polymerases I,II,and III subunit ABC5,DNA-directed RNA polymerase III subunit L | |
| 11 | K | DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC4 | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) In PDB | Sus scrofa (Pig) | |
| 12 | L | DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC4 | polymer | 58 | 7018.2 | 1 | UniProt (I3LN51) Pfam (PF03604) UniProt (by SIFTS) (A0A4X1TRS6) In PDB | Sus scrofa (Pig) | |
| 13 | M | AMATOXIN | polymer | 8 | 939.0 | 1 | UniProt (P85421) In PDB BIRD (PRD_000201) | Amanita phalloides | |
| 14 | N | DNA (25-MER) | polymer | 43 | 13303.6 | 1 | synthetic construct | ||
| 15 | P | RNA (5'-R(P*CP*AP*UP*AP*AP*AP*GP*AP*CP*CP*AP*GP*GP*C)-3') | polymer | 20 | 6414.9 | 1 | synthetic construct | ||
| 16 | T | DNA (36-MER) | polymer | 43 | 13178.5 | 1 | synthetic construct | ||
| 17 | A, B, C, I, J... | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 18 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 1970 (UniProt: I3LJR4)
| PDB | External Database | Details |
|---|---|---|
| Val 526 | - | insertion |
| Thr 527 | - | insertion |
| Pro 528 | - | insertion |
| Gln 529 | - | insertion |
| Gln 1299 | - | insertion |
| Gly 1300 | - | insertion |
| Ile 1301 | - | insertion |
| Glu 1302 | - | insertion |
| Gln 1303 | - | insertion |
| Ile 1304 | - | insertion |
| Ser 1305 | - | insertion |
| Tyr 1308 | Ser 1297 | conflict |
| Met 1309 | Arg 1298 | conflict |
| His 1310 | Ser 1299 | conflict |
| Gln 1313 | Ala 1302 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 550380.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 547.6 | |
| All* | Total formula weight | 550928.3 |
