6ESI

Nucleosome breathing : Class 4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D (D)	Histone H2B 1.1	polymer	122	13552.8	1	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	H (H)	Histone H2B 1.1	polymer	122	13524.8	1	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
6	I (I)	DNA (133-MER)	polymer	147	45604.0	1		synthetic construct
7	J (J)	DNA (133-MER)	polymer	147	45145.8	1		synthetic construct

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	variant

D: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	variant
Arg 122	Lys 126	variant

H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	variant

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	198918.1
All*	Total formula weight	198918.1

*Water molecules are not included.