6ENF
Cryo-EM structure of a polyproline-stalled ribosome in the absence of EF-P
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (a) | 16S ribosomal RNA | polymer | 1539 | 498725.4 | 1 | GenBank (1114277939) | Escherichia coli | |
| 2 | B (b) | 30S ribosomal protein S2 | polymer | 218 | 24253.9 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli | Small ribosomal subunit protein uS2 |
| 3 | C (c) | 30S ribosomal protein S3 | polymer | 206 | 23078.8 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli | Small ribosomal subunit protein uS3 |
| 4 | D (d) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli | Small ribosomal subunit protein uS4 |
| 5 | E (e) | 30S ribosomal protein S5 | polymer | 157 | 16532.1 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli | Small ribosomal subunit protein uS5 |
| 6 | F (f) | 30S ribosomal protein S6 | polymer | 100 | 11669.4 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli | Small ribosomal subunit protein bS6 |
| 7 | G (g) | 30S ribosomal protein S7 | polymer | 151 | 16861.5 | 1 | UniProt (P02359) Pfam (PF00177) | Escherichia coli | Small ribosomal subunit protein uS7 |
| 8 | H (h) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli | Small ribosomal subunit protein uS8 |
| 9 | I (i) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli | Small ribosomal subunit protein uS9 |
| 10 | J (j) | 30S ribosomal protein S10 | polymer | 98 | 11197.0 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli | Small ribosomal subunit protein uS10 |
| 11 | K (k) | 30S ribosomal protein S11 | polymer | 116 | 12388.1 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli | Small ribosomal subunit protein uS11 |
| 12 | L (l) | 30S ribosomal protein S12 | polymer | 123 | 13637.0 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli | Small ribosomal subunit protein uS12 |
| 13 | M (m) | 30S ribosomal protein S13 | polymer | 114 | 12625.8 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli | Small ribosomal subunit protein uS13 |
| 14 | N (n) | 30S ribosomal protein S14 | polymer | 101 | 11546.4 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli | Small ribosomal subunit protein uS14 |
| 15 | O (o) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli | Small ribosomal subunit protein uS15 |
| 16 | P (p) | 30S ribosomal protein S16 | polymer | 82 | 9207.6 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli | Small ribosomal subunit protein bS16 |
| 17 | Q (q) | 30S ribosomal protein S17 | polymer | 80 | 9263.9 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli | Small ribosomal subunit protein uS17 |
| 18 | R (r) | 30S ribosomal protein S18 | polymer | 65 | 7606.8 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli | Small ribosomal subunit protein bS18 |
| 19 | S (s) | 30S ribosomal protein S19 | polymer | 79 | 9057.6 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli | Small ribosomal subunit protein uS19 |
| 20 | T (t) | 30S ribosomal protein S20 | polymer | 85 | 9506.2 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli | Small ribosomal subunit protein bS20 |
| 21 | U (u) | 30S ribosomal protein S21 | polymer | 65 | 7763.1 | 1 | UniProt (P68679) Pfam (PF01165) | Escherichia coli | Small ribosomal subunit protein bS21 |
| 22 | V (v) | RNA (5'-R(P*CP*CP*G)-3'), mRNA | polymer | 3 | 910.6 | 1 | Escherichia coli | ||
| 23 | W (A) | 23S ribosomal RNA | polymer | 2903 | 941305.3 | 1 | GenBank (802133627) | Escherichia coli | |
| 24 | X (B) | 5S ribosomal RNA | polymer | 120 | 38813.1 | 1 | GenBank (1213441078) | Escherichia coli | |
| 25 | Y (C) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli | Large ribosomal subunit protein uL2 |
| 26 | Z (D) | 50S ribosomal protein L3 | polymer | 209 | 22277.5 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli | Large ribosomal subunit protein uL3 |
| 27 | AA (E) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli | Large ribosomal subunit protein uL4 |
| 28 | BA (F) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli | Large ribosomal subunit protein uL5 |
| 29 | CA (G) | 50S ribosomal protein L6 | polymer | 176 | 18801.6 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli | Large ribosomal subunit protein uL6 |
| 30 | DA (J) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli | Large ribosomal subunit protein uL13 |
| 31 | EA (K) | 50S ribosomal protein L14 | polymer | 122 | 13451.9 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli | Large ribosomal subunit protein uL14 |
| 32 | FA (L) | 50S ribosomal protein L15 | polymer | 143 | 14877.3 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli | Large ribosomal subunit protein uL15 |
| 33 | GA (M) | 50S ribosomal protein L16 | polymer | 136 | 15312.3 | 1 | UniProt (P0ADY7) Pfam (PF00252) | Escherichia coli | Large ribosomal subunit protein uL16 |
| 34 | HA (N) | 50S ribosomal protein L17 | polymer | 120 | 13721.9 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli | Large ribosomal subunit protein bL17 |
| 35 | IA (O) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli | Large ribosomal subunit protein uL18 |
| 36 | JA (P) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli | Large ribosomal subunit protein bL19 |
| 37 | KA (Q) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli | Large ribosomal subunit protein bL20 |
| 38 | LA (R) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli | Large ribosomal subunit protein bL21 |
| 39 | MA (S) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli | Large ribosomal subunit protein uL22 |
| 40 | NA (T) | 50S ribosomal protein L23 | polymer | 93 | 10546.5 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli | Large ribosomal subunit protein uL23 |
| 41 | OA (U) | 50S ribosomal protein L24 | polymer | 102 | 11078.9 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli | Large ribosomal subunit protein uL24 |
| 42 | PA (V) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli | Large ribosomal subunit protein bL25 |
| 43 | QA (W) | 50S ribosomal protein L27 | polymer | 78 | 8419.6 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli | Large ribosomal subunit protein bL27 |
| 44 | RA (X) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli | Large ribosomal subunit protein bL28 |
| 45 | SA (Y) | 50S ribosomal protein L29 | polymer | 63 | 7286.5 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli | Large ribosomal subunit protein uL29 |
| 46 | TA (Z) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli | Large ribosomal subunit protein uL30 |
| 47 | UA (0) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli | Large ribosomal subunit protein bL32 |
| 48 | VA (1) | 50S ribosomal protein L33 | polymer | 50 | 5814.8 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli | Large ribosomal subunit protein bL33 |
| 49 | WA (2) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli | Large ribosomal subunit protein bL34 |
| 50 | XA (3) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 51 | YA (4) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 52 | ZA (6) | 50S ribosomal protein L31 | polymer | 66 | 7516.7 | 1 | UniProt (P0A7M9) Pfam (PF01197) | Escherichia coli | Large ribosomal subunit protein bL31-A |
| 53 | AB (x) | proline tRNA | polymer | 77 | 24846.7 | 1 | GenBank (1231762938) | Escherichia coli |
Sequence modifications
n: 1 - 101 (UniProt: P0AG59)
A: 1 - 2903 (GenBank: 802133627)
B: 1 - 120 (GenBank: 1213441078)
| PDB | External Database | Details |
|---|---|---|
| Ala 35 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| C 747 | U 2724742 | conflict |
| G 1847 | A 2723642 | conflict |
| A 2069 | G 2723420 | conflict |
| PDB | External Database | Details |
|---|---|---|
| A 120 | U 4143480 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 53 |
| Total formula weight | 2122173.8 | |
| All* | Total formula weight | 2122173.8 |
