6EMS

Crystal Structure of dual specific Trm10 construct from Thermococcus kodakaraensis.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (guanine(9)-/adenine(9)-N1)-methyltransferase	polymer	364	42217.8	1	UniProt (Q5JD38)	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis)	tRNA(m1G9/m1A9)-methyltransferase,tRNA(m1G9/m1A9)MTase
2	B (A)	water	water		18.0	65	Chemie (HOH)

Sequence modifications

A: 1 - 344 (UniProt: Q5JD38)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 77	Cys 77	engineered mutation
Ala 120	Cys 120	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	42217.8
All*	Total formula weight	42217.8

*Water molecules are not included.