6EI9

Crystal structure of E. coli tRNA-dihydrouridine synthase B (DusB)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	tRNA-dihydrouridine synthase B	polymer	340	37945.3	2	UniProt (P0ABT7) Pfam (PF01207) UniProt (by SIFTS) (P0ABT5) In PDB	Escherichia coli
2	A, B	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	A, B	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	A, B	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
5	B	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
6		water	water		18.0	140	Chemie (HOH)

Sequence modifications

A, B: 1 - 321 (UniProt: P0ABT7)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75890.6
Non-Polymers*	Number of molecules	13
	Total formula weight	2103.7
All*	Total formula weight	77994.3

*Water molecules are not included.