6EDT

Mycobacterium tuberculosis RNAP open promoter complex with RbpA/CarD and AP3 promoter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	347	37745.3	2	UniProt (A5U8D3) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P9WGZ1)	Mycobacterium tuberculosis	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1134	125390.9	1	UniProt (V9Z879) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) UniProt (by SIFTS) (P9WGY9)	Mycobacterium tuberculosis	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1371	152882.1	1	UniProt (A5U053) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) UniProt (by SIFTS) (P9WGY7)	Mycobacterium tuberculosis	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	110	11777.0	1	UniProt (A0A0T9N9K3) Pfam (PF01192) UniProt (by SIFTS) (P9WGY5)	Mycobacterium tuberculosis	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor SigA	polymer	531	58169.5	1	UniProt (P9WGI0) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P9WGI1)	Mycobacterium tuberculosis	Sigma-A
6	G (J)	RNA polymerase-binding protein RbpA	polymer	111	12993.7	1	UniProt (P9WHJ4) Pfam (PF13397) UniProt (by SIFTS) (P9WHJ5)	Mycobacterium tuberculosis
7	H (O)	DNA (65-MER)	polymer	90	27907.8	1		Mycobacterium tuberculosis
8	I (P)	DNA (65-MER)	polymer	90	27618.6	1		Mycobacterium tuberculosis
9	J (M)	RNA polymerase-binding transcription factor CarD	polymer	162	17933.4	1	UniProt (P9WJG2) Pfam (PF02559) Pfam (PF21095) UniProt (by SIFTS) (P9WJG3)	Mycobacterium tuberculosis
10	K, L (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
11	M (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1316 (UniProt: A5U053)

PDB	External Database	Details
Asp -46	-	expression tag
Gly -45	-	expression tag
Ala -44	-	expression tag
Ala -43	-	expression tag
Ile -42	-	expression tag
Glu -41	-	expression tag
Leu -40	-	expression tag
Arg -39	-	expression tag
Glu -38	-	expression tag
Gly -37	-	expression tag
Glu -36	-	expression tag
Asp -35	-	expression tag
Glu -34	-	expression tag
Asp -33	-	expression tag
Leu -32	-	expression tag
Glu -31	-	expression tag
Arg -30	-	expression tag
Ala -29	-	expression tag
Ala -28	-	expression tag
Ala -27	-	expression tag
Asn -26	-	expression tag
Leu -25	-	expression tag
Gly -24	-	expression tag
Ile -23	-	expression tag
Asn -22	-	expression tag
Leu -21	-	expression tag
Ser -20	-	expression tag
Arg -19	-	expression tag
Asn -18	-	expression tag
Glu -17	-	expression tag
Ser -16	-	expression tag
Ala -15	-	expression tag
Ser -14	-	expression tag
Val -13	-	expression tag
Glu -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Ala -9	-	expression tag
Leu -8	-	expression tag
Ala -7	-	expression tag
Arg -6	-	expression tag
His -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ala 0	-	expression tag
His 1317	-	expression tag
His 1318	-	expression tag
His 1319	-	expression tag
His 1320	-	expression tag
His 1321	-	expression tag
His 1322	-	expression tag
His 1323	-	expression tag
His 1324	-	expression tag

E: 2 - 110 (UniProt: A0A0T9N9K3)

PDB	External Database	Details
Gly 1	-	expression tag

F: 1 - 528 (UniProt: P9WGI0)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	510163.6
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	510318.7

*Water molecules are not included.