6E3Y

Cryo-EM structure of the active, Gs-protein complexed, human CGRP receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (P)	Calcitonin gene-related peptide 1	polymer	38	3795.4	1	UniProt (P06881)	Homo sapiens	Alpha-type CGRP,Calcitonin gene-related peptide I,CGRP-I
2	B (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama (llama)
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
6	F (R)	Calcitonin gene-related peptide type 1 receptor	polymer	490	56274.5	1	UniProt (Q16602) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (Human)	CGRP type 1 receptor,Calcitonin receptor-like receptor
7	G (E)	Receptor activity-modifying protein 1	polymer	149	17066.7	1	UniProt (O60894) Pfam (PF04901)	Homo sapiens (Human)	Calcitonin-receptor-like receptor activity-modifying protein 1,CRLR activity-modifying protein 1

Sequence modifications

P: 1 - 37 (UniProt: P06881)

PDB	External Database	Details
Nh2 38	-	amidation

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 22 - 461 (UniProt: Q16602)

PDB	External Database	Details
Met -9	-	initiating methionine
Lys -8	-	expression tag
Thr -7	-	expression tag
Ile -6	-	expression tag
Ile -5	-	expression tag
Ala -4	-	expression tag
Leu -3	-	expression tag
Ser -2	-	expression tag
Tyr -1	-	expression tag
Ile 0	-	expression tag
Phe 1	-	expression tag
Cys 2	-	expression tag
Leu 3	-	expression tag
Val 4	-	expression tag
Phe 5	-	expression tag
Ala 6	-	expression tag
Asp 7	-	expression tag
Tyr 8	-	expression tag
Lys 9	-	expression tag
Asp 10	-	expression tag
Asp 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Leu 14	-	expression tag
Glu 15	-	expression tag
Val 16	-	expression tag
Leu 17	-	expression tag
Phe 18	-	expression tag
Gln 19	-	expression tag
Gly 20	-	expression tag
Pro 21	-	expression tag
Pro 462	-	expression tag
Ala 463	-	expression tag
Gly 464	-	expression tag
Leu 465	-	expression tag
Glu 466	-	expression tag
Val 467	-	expression tag
Leu 468	-	expression tag
Phe 469	-	expression tag
Gln 470	-	expression tag
Gly 471	-	expression tag
Pro 472	-	expression tag
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag

E: 27 - 148 (UniProt: O60894)

PDB	External Database	Details
Met 0	-	initiating methionine
Lys 1	-	expression tag
Thr 2	-	expression tag
Ile 3	-	expression tag
Ile 4	-	expression tag
Ala 5	-	expression tag
Leu 6	-	expression tag
Ser 7	-	expression tag
Tyr 8	-	expression tag
Ile 9	-	expression tag
Phe 10	-	expression tag
Cys 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Phe 14	-	expression tag
Ala 15	-	expression tag
Asp 16	-	expression tag
Tyr 17	-	expression tag
Lys 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Asp 21	-	expression tag
Asp 22	-	expression tag
Lys 23	-	expression tag
His 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	184356.0
All*	Total formula weight	184356.0

*Water molecules are not included.