6E0P
Cryo-EM structure of the centromeric nucleosome (Native alpha satellite DNA) in complex with a single chain antibody fragment
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, E | Histone H3-like centromeric protein A | polymer | 158 | 18038.8 | 2 | UniProt (P49450) Pfam (PF00125) In PDB | Homo sapiens (Human) | Centromere autoantigen A,Centromere protein A,CENP-A |
2 | B, F | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62805) Pfam (PF15511) In PDB | Homo sapiens (Human) | |
3 | C, G | Histone H2A type 1-B/E | polymer | 130 | 14165.6 | 2 | UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB | Homo sapiens (Human) | Histone H2A.2,Histone H2A/a,Histone H2A/m |
4 | D, H | Histone H2B type 1-J | polymer | 126 | 13935.2 | 2 | UniProt (P06899) Pfam (PF00125) In PDB | Homo sapiens (Human) | Histone H2B.1,Histone H2B.r,H2B/r |
5 | I | DNA (145-MER) | polymer | 145 | 44539.5 | 1 | Homo sapiens | ||
6 | J | DNA (145-MER) | polymer | 145 | 44948.8 | 1 | Homo sapiens | ||
7 | M, N | scFv | polymer | 265 | 29030.1 | 2 | Mus musculus (house mouse) |
Sequence modifications
A, E: 1 - 140 (UniProt: P49450)
PDB | External Database | Details |
---|---|---|
Met -17 | - | initiating methionine |
Gly -16 | - | expression tag |
Ser -15 | - | expression tag |
Ser -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
Ser -7 | - | expression tag |
Ser -6 | - | expression tag |
Gly -5 | - | expression tag |
Leu -4 | - | expression tag |
Pro -3 | - | expression tag |
Arg -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 12 |
Total formula weight | 262616.7 | |
All* | Total formula weight | 262616.7 |