6E0P

Cryo-EM structure of the centromeric nucleosome (Native alpha satellite DNA) in complex with a single chain antibody fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3-like centromeric protein A	polymer	158	18038.8	2	UniProt (P49450) Pfam (PF00125) In PDB	Homo sapiens (Human)	Centromere autoantigen A,Centromere protein A,CENP-A
2	B, F	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1-B/E	polymer	130	14165.6	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-J	polymer	126	13935.2	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I	DNA (145-MER)	polymer	145	44539.5	1		Homo sapiens
6	J	DNA (145-MER)	polymer	145	44948.8	1		Homo sapiens
7	M, N	scFv	polymer	265	29030.1	2		Mus musculus (house mouse)

Sequence modifications

A, E: 1 - 140 (UniProt: P49450)

PDB	External Database	Details
Met -17	-	initiating methionine
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Leu -4	-	expression tag
Pro -3	-	expression tag
Arg -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	262616.7
All*	Total formula weight	262616.7

*Water molecules are not included.