6DZT

Cryo-EM structure of nucleosome in complex with a single chain antibody fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3	polymer	136	15421.1	2	UniProt (P02299) Pfam (PF00125) In PDB	Drosophila melanogaster (Fruit fly)
2	B, F	Histone H4	polymer	104	11521.6	2	UniProt (P84040) Pfam (PF15511) In PDB	Drosophila melanogaster (Fruit fly)
3	C, G	Histone H2A	polymer	124	13388.7	2	UniProt (P84051) Pfam (PF00125) Pfam (PF16211) In PDB	Drosophila melanogaster (Fruit fly)
4	D, H	Histone H2B	polymer	124	13840.2	2	UniProt (P02283) Pfam (PF00125) In PDB	Drosophila melanogaster (Fruit fly)
5	I	DNA (147-MER)	polymer	147	45610.0	1		Escherichia coli
6	J	DNA (147-MER)	polymer	147	45138.8	1		Escherichia coli
7	M, N	scFv	polymer	265	29030.1	2		Mus musculus (house mouse)

Sequence modifications

B, F: 1 - 102 (UniProt: P84040)

PDB	External Database	Details
Met -1	-	initiating methionine
Ile 0	-	expression tag

D, H: 1 - 122 (UniProt: P02283)

PDB	External Database	Details
Met -1	-	initiating methionine
Ile 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	257152.4
All*	Total formula weight	257152.4

*Water molecules are not included.