6DYK

Iron- and Nitric Oxide-bound structure of the engineered cyt b562 variant, CH3Y*

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, C, E, G)	Soluble cytochrome b562	polymer	106	11930.4	4	UniProt (P0ABE7) Pfam (PF07361)	Escherichia coli	Cytochrome b-562
2	E, G (E, G)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
3	F, H (E, G)	NITRIC OXIDE	non-polymer		30.0	2	Chemie (NO)
4	I, J, K, L (A, C, E, G)	water	water		18.0	238	Chemie (HOH)

Sequence modifications

A, C, E, G: 1 - 106 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 7	Met 29	engineered mutation
Trp 59	Lys 81	engineered mutation
His 67	Ile 89	engineered mutation
Tyr 70	Gly 92	engineered mutation
His 71	Gln 93	engineered mutation
Cys 96	Thr 118	engineered mutation
His 97	Thr 119	engineered mutation
Ala 101	Tyr 123	engineered mutation
Ile 102	His 124	engineered mutation
Leu 106	Arg 128	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47721.5
Non-Polymers*	Number of molecules	4
	Total formula weight	171.7
All*	Total formula weight	47893.2

*Water molecules are not included.